Effect of centrifugation time on growth factor and MMP release of an experimental platelet-rich fibrin-type product

Simple item page
dc.contributor.authorEren G.
dc.contributor.authorGürkan A.
dc.contributor.authorAtmaca H.
dc.contributor.authorDönmez A.
dc.contributor.authorAtilla G.
dc.date.accessioned2024-07-22T08:11:35Z
dc.date.available2024-07-22T08:11:35Z
dc.date.issued2016
dc.description.abstractAbstract: Platelet-rich fibrin (PRF) has a controlled release of growth factors due to the fibrin matrix structure. Different centrifugation protocols were suggested for PRF preparation. Since the derivation method of PRF can alter its contents, in the present study it is aimed to investigate the cell contents and transforming growth factor beta-1 (TGF-β1), platelet-derived growth factor (PDGF-AB), vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-1 and-8 release from experimental PRF-type membranes obtained with different centrifugation times at 400 gravity. Three blood samples were collected from 20 healthy non-smoker volunteers. One tube was used for whole blood analyses. The other two tubes were centrifuged at 400 g for 10 minutes (group A) or 12 minutes (group B). Each experimental PRF-type membrane was placed in Dulbecco’s Modified Eagle’s Medium (DMEM)and at 1, 24 and 72 hours, TGF-β1, PDGF-AB, VEGF, MMP-1 and -8 release amounts were analysed by enzyme-linked immunosorbent assay (ELISA). The blood cell count of membranes was determined by subtracting plasma supernatant and red blood cell (RBC) mixture from the whole blood cell counts. At 72 hours, the VEGF level of group B was statistically higher than that of group A (p = 0.040). The centrifugation time was not found to influence the release of other growth factors, enzymes and cell counts. Within the limits of the present study, it might be suggested that centrifugation time at a constant gravity has a significant effect on the VEGF levels released from experimental PRF-type membrane. It can be concluded that due to the importance of VEGF in the tissue healing process, membranes obtained at 12-minute centrifugation time may show a superior potential in wound healing. © 2016 Taylor & Francis.
dc.identifier.DOI-ID10.3109/09537104.2015.1131253
dc.identifier.issn09537104
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15712
dc.language.isoEnglish
dc.publisherTaylor and Francis Ltd
dc.subjectAdult
dc.subjectBiomarkers
dc.subjectBlood Cell Count
dc.subjectBlood Platelets
dc.subjectCase-Control Studies
dc.subjectCentrifugation
dc.subjectFemale
dc.subjectFibrin
dc.subjectHumans
dc.subjectIntercellular Signaling Peptides and Proteins
dc.subjectMale
dc.subjectMatrix Metalloproteinases
dc.subjectPeriodontal Diseases
dc.subjectPlatelet-Rich Plasma
dc.subjectYoung Adult
dc.subjectfibrin
dc.subjectgrowth factor
dc.subjectinterstitial collagenase
dc.subjectneutrophil collagenase
dc.subjectplatelet derived growth factor AB
dc.subjectplatelet rich fibrin
dc.subjecttransforming growth factor beta1
dc.subjectunclassified drug
dc.subjectvasculotropin
dc.subjectbiological marker
dc.subjectfibrin
dc.subjectmatrix metalloproteinase
dc.subjectsignal peptide
dc.subjectadult
dc.subjectArticle
dc.subjectblood cell count
dc.subjectblood sampling
dc.subjectcellular distribution
dc.subjectcentrifugation
dc.subjectcontrolled study
dc.subjectenzyme linked immunosorbent assay
dc.subjecterythrocyte
dc.subjectfemale
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman experiment
dc.subjectleukocyte count
dc.subjectmale
dc.subjectnormal human
dc.subjectpriority journal
dc.subjectthrombocyte count
dc.subjectthrombocyte rich plasma
dc.subjecttime
dc.subjectblood
dc.subjectcase control study
dc.subjectmetabolism
dc.subjectperiodontal disease
dc.subjectthrombocyte
dc.subjectyoung adult
dc.titleEffect of centrifugation time on growth factor and MMP release of an experimental platelet-rich fibrin-type product
dc.typeArticle

Files

Collections

Scopus Koleksiyonu