Effects of a DNA Demethylating Agent - 5-Azacytidine - on Testicular Morphology during Mouse Embryo Development

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dc.contributor.authorBulut H.E.
dc.contributor.authorÖzdemir Ö.
dc.contributor.authorBaşimoglu-Koca Y.
dc.contributor.authorKorkmaz M.
dc.contributor.authorAtalay A.
dc.date.accessioned2024-07-22T08:25:54Z
dc.date.available2024-07-22T08:25:54Z
dc.date.issued1999
dc.description.abstractDNA methylation is an epigenetical mechanism that plays crucial roles in cellular differentiation and tissue development in embryogenesis. The aim of the present study was to determine the effects of a demethylating agent, 5-azacytidine, on testicular development during embryonal life in mouse. Ten pregnant mice were administered 5-azacytidine (5-azaC) (i.p 2mg/kg of agent dissolved in 0.1 mg/ml PBS) during 8th (Group 1), 11th (Group 2), 14th (Group 3) and 18th (Group 4) days of pregnancy periods and male siblings of these animals were obtained (experimental groups) whereas the control group animals received no treatment and siblings of this group were also obtained. Testicular tissues from all groups were taken 20 days after birth and examined at the light and electron microscopical levels. All pregnancies were terminated in Group 1 animals, therefore no observations could be done in this group. While Group 2 and 3 siblings showed distinctive kongenital abnormalities such as; anancephaly, growth failure, cleft palate, extremity abnormalities, supernumerary ribs and whirled shaped-tails, no such abnormalities were observed in Group 4 when compared to the control group. Microscopical examination of testicular tissues in groups 2 and 3 demonstrated cellular disintegration of spermatocytes in seminiferous tubules. In addition, cytoplasmic vacuoles and thickening of the basement membrane were also evident in both groups 2 and 3. Apoptotic-like cells were seen especially in group 2 and rarely in group 3. There were no structural alterations in group 4 animals, except a decreased number of spermatocytes in seminiferous tubules when compared to the control group, possibly indicating the completion of embryogenesis in this group. In conclusion, it could be suggested that the demethylating agent 5-azacytidine may trigger an unknown gene reactivation during early embryogenesis possibly affecting the cell and tissue differentiation in developing mammalian embryos.
dc.identifier.DOI-ID10.2535/ofaj1936.76.1_47
dc.identifier.issn0030154X
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/20665
dc.language.isoEnglish
dc.publisherOkajima Foria Anatomica Yaponika Henshubu
dc.rightsAll Open Access; Bronze Open Access
dc.subjectAnimals
dc.subjectAzacitidine
dc.subjectEmbryonic and Fetal Development
dc.subjectFemale
dc.subjectInjections, Intraperitoneal
dc.subjectMale
dc.subjectMice
dc.subjectMicroscopy, Electron
dc.subjectPregnancy
dc.subjectSeminiferous Tubules
dc.subjectTestis
dc.subjectazacitidine
dc.subjectanimal
dc.subjectarticle
dc.subjectdrug effect
dc.subjectelectron microscopy
dc.subjectfemale
dc.subjectintraperitoneal drug administration
dc.subjectmale
dc.subjectmouse
dc.subjectpathology
dc.subjectpregnancy
dc.subjectprenatal development
dc.subjectseminiferous tubule
dc.subjecttestis
dc.subjectultrastructure
dc.titleEffects of a DNA Demethylating Agent - 5-Azacytidine - on Testicular Morphology during Mouse Embryo Development
dc.typeArticle

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