Design of 99mTc radiolabeled gemcitabine polymeric nanoparticles as drug delivery system and in vivo evaluation

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dc.contributor.authorİçhedef Ç.
dc.contributor.authorTeksöz S.
dc.contributor.authorÇetin O.
dc.contributor.authorAydın B.
dc.contributor.authorSarıkavak İ.
dc.contributor.authorParlak Y.
dc.contributor.authorBilgin B.E.
dc.date.accessioned2024-07-22T08:06:16Z
dc.date.available2024-07-22T08:06:16Z
dc.date.issued2021
dc.description.abstractThe main objective of this study is to create a multifunctional drug carrier system as a prototype product. For this purpose, Gemcitabine that used as an anticancer agent for several kinds of tumour therapy, and technetium(I)-tricarbonyl core, [99mTc(CO)3]+, was incorporated to the polymeric structure. In the current study PLA-PEG-COOH (polylactic acid-polyethylene glycol-carboxyl) copolymer was synthesized and the structure analysis was performed by FT-IR and 1H NMR. The surface charge, size and morphology of the polymeric nanoparticles was evaluated by Zeta Potential (ZP) analysis, Dynamic Light Scattering (DLS) and Scanning Electron Microscopy (SEM) methods. Gemcitabine loaded polymeric nanoparticles were radiolabeled with [99mTc(CO)3(H2O)3]+ and quality controls were performed by thin layer radiochromatograpy (TLRC) and high performance liquid radiochromatograpy (HPLRC). PLA-PEG-GEM complex was labeled with [99mTc(CO)3]+ in high radiochemical yield and purity. The radiolabeled complex is stable during the study period in saline solution. The biological activity of this radiolabeled system was evaluated performing biodistribution and gamma camera imaging on Wistar Albino rats which exhibited higher uptake in the lung and liver at all selected time points. © 2021 Elsevier B.V.
dc.identifier.DOI-ID10.1016/j.matchemphys.2021.124380
dc.identifier.issn02540584
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13459
dc.language.isoEnglish
dc.publisherElsevier Ltd
dc.subjectBioactivity
dc.subjectControlled drug delivery
dc.subjectDynamic light scattering
dc.subjectMorphology
dc.subjectNanoparticles
dc.subjectPolyethylene glycols
dc.subjectPolyethylene oxides
dc.subjectPolyethylenes
dc.subjectScanning electron microscopy
dc.subjectTechnetium compounds
dc.subjectAnti-cancer agents
dc.subjectDrug carrier systems
dc.subjectDrug delivery system
dc.subjectPolymeric nanoparticles
dc.subjectPolymeric structures
dc.subjectRadiochemical yield
dc.subjectStructure analysis
dc.subjectZeta potential (zp)
dc.subjectQuality control
dc.titleDesign of 99mTc radiolabeled gemcitabine polymeric nanoparticles as drug delivery system and in vivo evaluation
dc.typeArticle

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