Investigation of therapeutic efficiency of bleomycin and bleomycin-glucuronide labeled with 131I on the cancer cell lines

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dc.contributor.authorEdiz M.
dc.contributor.authorAvcibaşi U.
dc.contributor.authorÜnak P.
dc.contributor.authorMüftüler F.Z.B.
dc.contributor.authorMedine E.I.
dc.contributor.authorYurt Kilçar A.
dc.contributor.authorDemiroǧlu H.
dc.contributor.authorGümüşer F.G.
dc.contributor.authorSakarya S.
dc.date.accessioned2024-07-22T08:18:34Z
dc.date.available2024-07-22T08:18:34Z
dc.date.issued2013
dc.description.abstractThe aim of this study is to determine the incorporations of radiolabeled bleomycin (131I-BLM) and bleomycin-glucuronide (131I- BLMGLU) on PC-3 (human prostate carcinoma cell line), Caco-2 (human colon adenocarcinoma cell line), Hutu-80 (Human Duodenum adenocarcinoma cell line), and A549 (Human lung adenocarcinoma epithelial cell line) cancerous cell lines. For this purpose, BLM and BLMGLU enyzmatically synthesized were labeled with 131I, quality control studies were done and the incorporation yields of 131I-BLM and 131I-BLMGLU on these cell lines were measured. Quality-control studies showed that the radiolabeling yields were obtained as 95% and 90% for 131I-BLM and 131I-BLMGLU, respectively. Also, as a result of the cell culture studies, it was found that 131I-BLM and 131I-BLMGLU had higher incorporation on PC-3 cells than that of other cell lines. In addition to this, it was reported that the incorporation yield of 131I-BLMGLU was higher than that 131I-BLM. At the end of the study, cytotoxicities of BLM and BLMGLU on PC-3 cancerous cell line were inspected and fluorescent images of BLM and BLMGLU were taken on PC-3 cells by using fluorescein isothiocyanate. In conclusion, cell culture studies demonstrated that the incorporation values of 131I-BLMGLU on the four cell lines were about five to six times higher than 131I-BLM. Radiolabeled glucuronide derivatives can be used in cancer therapy and tumor imaging, depending on the properties of radioiodine for the β-glucuronidase-rich tissues because glucuronidation leads to rapid and higher incorporation on adenocarcinoma cells. © Mary Ann Liebert, Inc.
dc.identifier.DOI-ID10.1089/cbr.2012.1316
dc.identifier.issn10849785
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17366
dc.language.isoEnglish
dc.publisherMary Ann Liebert Inc.
dc.subjectAdenocarcinoma
dc.subjectAntineoplastic Agents
dc.subjectApoptosis
dc.subjectBleomycin
dc.subjectCaco-2 Cells
dc.subjectCell Proliferation
dc.subjectDuodenal Neoplasms
dc.subjectGlucuronides
dc.subjectHumans
dc.subjectIodine Radioisotopes
dc.subjectLung Neoplasms
dc.subjectMale
dc.subjectOptical Imaging
dc.subjectProstatic Neoplasms
dc.subjectbleomycin
dc.subjectbleomycin glucuronide i 131
dc.subjectbleomycin i 131
dc.subjectfluorescein isothiocyanate
dc.subjectglucuronide
dc.subjectradiopharmaceutical agent
dc.subjectunclassified drug
dc.subjectantineoplastic agent
dc.subjectbleomycin
dc.subjectglucuronide
dc.subjectradioactive iodine
dc.subjectanimal tissue
dc.subjectantineoplastic activity
dc.subjectapoptosis
dc.subjectarticle
dc.subjectcancer cell culture
dc.subjectcell survival
dc.subjectcell viability
dc.subjectcolon adenocarcinoma
dc.subjectconcentration response
dc.subjectcontrolled study
dc.subjectdiagnostic imaging
dc.subjectdrug glucuronidation
dc.subjectdrug structure
dc.subjectdrug synthesis
dc.subjecthigh performance liquid chromatography
dc.subjecthuman
dc.subjecthuman cell
dc.subjectin vitro study
dc.subjectisotope labeling
dc.subjectlung adenocarcinoma
dc.subjectmale
dc.subjectmicrosome
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectprostate carcinoma
dc.subjectquality control
dc.subjectradiation injury
dc.subjectradiochromatography
dc.subjectradioiodination
dc.subjectrat
dc.subjectstomach adenocarcinoma
dc.subjectadenocarcinoma
dc.subjectCACO 2 cell line
dc.subjectcell proliferation
dc.subjectchemistry
dc.subjectdrug effects
dc.subjectduodenum tumor
dc.subjectfluorescence imaging
dc.subjectlung tumor
dc.subjectmetabolism
dc.subjectProstatic Neoplasms
dc.titleInvestigation of therapeutic efficiency of bleomycin and bleomycin-glucuronide labeled with 131I on the cancer cell lines
dc.typeArticle

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