Early renal parenchymal histological changes in an experimental model of vesico-ureteral reflux and the role of apoptosis

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dc.contributor.authorŞencan A.
dc.contributor.authorVatansever S.
dc.contributor.authorYilmaz Ö.
dc.contributor.authorGenç A.
dc.contributor.authorSerter S.
dc.contributor.authorGümüser G.
dc.contributor.authorKurutepe S.
dc.contributor.authorPekindil G.
dc.contributor.authorGünsar C.
dc.contributor.authorMir E.
dc.date.accessioned2024-07-22T08:22:15Z
dc.date.available2024-07-22T08:22:15Z
dc.date.issued2008
dc.description.abstractObjectives. To observe early renal parenchymal cellular changes in an experimental model of vesico-ureteral reflux (VUR) and to show whether the apoptotic pathway plays a role in these cellular changes. Material and methods. Fourteen New Zealand breed rabbits were used and were divided into two equal groups (control and experimental groups). Urine samples were obtained in a sterile manner and cultured. In the study group, reflux was created in the right kidneys surgically. Renal scintigraphy and voiding cystourethrography (VCUG) were performed in both groups on Day 17. The kidneys were examined in terms of histology, apoptotic activity and caspase activity. Results. No growth was observed in urine cultures in either group. VUR was manifested in only two rabbits in the experimental group on VCUG. On renal scintigraphy, no renal scarring was observed in either of the groups and renal uptake values were in the normal range. There was a greater increase in collagen in the right kidneys in the experimental group than in the control group and apoptotic activity was significantly increased in the study group: 0% in the control group, 10.8%±0.7% in the experimental group (p<0.001). Caspase-6 activity was strongly positive and caspase-8 and -9 activities were moderately positive in the right kidneys of the experimental group. Caspase-6 activity was moderately positive, and caspase-8 and -9 activities were weakly positive in the contralateral kidneys of the experimental group. Caspase activities in the control group were negative (p<0.001). Conclusions. In this experimental model of VUR, apoptotic activity was initiated via the caspase-8 and -9 pathway and collagen tissue increased in the renal parenchyma where reflux occurred. The balance of apoptotic activity may play a key role in the occurrence of reflux nephropathy. © 2008 Taylor & Francis.
dc.identifier.DOI-ID10.1080/00365590701701632
dc.identifier.issn16512065
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18984
dc.language.isoEnglish
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectCaspase 10
dc.subjectCaspase 6
dc.subjectCaspase 8
dc.subjectDisease Models, Animal
dc.subjectFemale
dc.subjectImmunohistochemistry
dc.subjectIn Situ Nick-End Labeling
dc.subjectKidney
dc.subjectRabbits
dc.subjectSuccimer
dc.subjectVesico-Ureteral Reflux
dc.subjectcaspase 6
dc.subjectcaspase 8
dc.subjectcaspase 9
dc.subjectcollagen
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectapoptosis
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectenzyme activity
dc.subjectkidney parenchyma
dc.subjectkidney scintiscanning
dc.subjectmicturition cystourethrography
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjecturinalysis
dc.subjectvesicoureteral reflux
dc.titleEarly renal parenchymal histological changes in an experimental model of vesico-ureteral reflux and the role of apoptosis
dc.typeArticle

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