Methylphenidate has dose-dependent negative effects on rat spermatogenesis: Decreased round spermatids and testicular weight and increased p53 expression and apoptosis

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dc.contributor.authorCansu A.
dc.contributor.authorEkinci Ö.
dc.contributor.authorEkinci Ö.
dc.contributor.authorSerdaroglu A.
dc.contributor.authorErdǒan D.
dc.contributor.authorCo̧kun Z.K.
dc.contributor.authorGürgen S.G.
dc.date.accessioned2024-07-22T08:19:55Z
dc.date.available2024-07-22T08:19:55Z
dc.date.issued2011
dc.description.abstractIn the present study, we aimed to evaluate the possible effects of methylphenidate on rat testes. Forty-two Wistar rats were randomly distributed into three experimental groups of 14 rats each. For 90 days, each group via gavage received the following: group 1 = tap water (control group), group 2 = 5 mg/kg/day of ritalin (methylphenidate, MPH), and group 3 = 10 mg/kg/day of ritalin. After sacrificing the animals, the body weights as well as the absolute and relative testicular weights were measured. Testes were sampled, fixed, and processed and, by histopathological examination, quantitative morphometric analysis of Sertoli cells, spermatocytes, and spermatids was performed in stages II, V, and XII. Immunohistochemistry was performed for transforming growth factor (TGF)-β1 and p 53, and the apoptotic index was assessed through the TUNEL method. Group 2 had a reduction of round spermatids in stage II. Group 3 had reduction in both stage II and stage V spermatids, as well as lower testicular weight. The p 53 expression was increased in group 3. In groups 2 and 3, the TGF-β1 expression was reduced and the apoptotic index by TUNEL was increased. Body weights remained stable on either group. Our results showed that methylphenidate might negatively affect spermatogenesis not only by reducing testicular weight and amount of round spermatids but also by increasing apoptotic death and p 53 activation. The findings of the study, however, must be cautiously interpreted. © SAGE Publications 2011.
dc.identifier.DOI-ID10.1177/0960327110394224
dc.identifier.issn14770903
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17899
dc.language.isoEnglish
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectBody Weight
dc.subjectCentral Nervous System Stimulants
dc.subjectDopamine Uptake Inhibitors
dc.subjectMale
dc.subjectMethylphenidate
dc.subjectOrgan Size
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectSpermatogenesis
dc.subjectSpermatozoa
dc.subjectTestis
dc.subjectTransforming Growth Factor beta1
dc.subjectTumor Suppressor Protein p53
dc.subjectAnimalia
dc.subjectRattus
dc.subjectRattus norvegicus
dc.subjectmethylphenidate
dc.subjectprotein p53
dc.subjecttap water
dc.subjecttransforming growth factor beta1
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectapoptosis
dc.subjectarticle
dc.subjectbody weight
dc.subjectcell cycle
dc.subjectcontrolled study
dc.subjectdrug megadose
dc.subjecthistopathologic skin reaction
dc.subjectimmunohistochemistry
dc.subjectlow drug dose
dc.subjectmale
dc.subjectnick end labeling
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectrat
dc.subjectSertoli cell
dc.subjectspermatid
dc.subjectspermatocyte
dc.subjectspermatogenesis
dc.subjecttestis weight
dc.subjectWistar rat
dc.titleMethylphenidate has dose-dependent negative effects on rat spermatogenesis: Decreased round spermatids and testicular weight and increased p53 expression and apoptosis
dc.typeArticle

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