A mass screening survey of cystic echinococcosis by ultrasonography, Western blotting, and ELISA among university students in Manisa, Turkey

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dc.contributor.authorKilimcioǧlu A.A.
dc.contributor.authorGirginkardeşler N.
dc.contributor.authorKorkmaz M.
dc.contributor.authorÖzkol M.
dc.contributor.authorDüzgün F.
dc.contributor.authorÖstan T.
dc.contributor.authorPabuşcu Y.
dc.contributor.authorDinç G.
dc.contributor.authorOk T.Z.
dc.date.accessioned2024-07-22T08:17:50Z
dc.date.available2024-07-22T08:17:50Z
dc.date.issued2013
dc.description.abstractCystic echinococcosis (CE) is one of the most important zoonotic diseases in a wide geographic area, including Turkey. In the present project, a total of 4275 students from Celal Bayar University, Manisa, Turkey, were screened by ultrasonography (US) and specific antibodies for CE were examined by Western blotting (WB) and ELISA in finger prick blood samples of 2034 of 4275 volunteered students. We aimed to report the apparent prevalence of CE based on different diagnostic procedures and to compare WB and ELISA with US in diagnosis of CE in a mass screening setting. Six new cases were diagnosed as CE by US during the survey. In addition to these cases, three students were also detected to have been previously operated and pathologically confirmed for hepatic CE. US revealed parenchymal changes in these cases in concordance with their operation history; so, the prevalence of CE by US was calculated as 0.21% (9/4275) (95%CI, 0.11-0.39%) among university students in Manisa. Bands were detected at 8, 28, 32, 38, 42, 47, 70 and 90. kDa by WB and the cases were considered to be positive for CE when at least three of the bands were seen together. Apparent prevalence of CE by ELISA and WB were found to be 2.11% (43/2034) (95%CI, 1.57-2.83%) and 0.25% (5/2034) (95%CI, 0.10-0.57%), respectively. Of the six US positive cases, WB was positive in only one case with two cysts in the liver. All of four cases with liver involvement were positive by ELISA. The high prevalence of CE among university students in Manisa indicated that CE is a major health problem in this area of Turkey. Our results supported that WB is rather difficult and not feasible as a mass screening test and may not be effective for confirmation especially in asymptomatic cases. As a result, we recommend US to be used initially in mass screening surveys for CE followed by confirmation by ELISA for suspected cases. Further examination primarily by chest X-ray followed by computed tomography and/or magnetic resonance imaging, if needed, should be recommended for US negative, ELISA and WB positive individuals who may have non-abdominal cysts. © 2013 Elsevier B.V.
dc.identifier.DOI-ID10.1016/j.actatropica.2013.08.010
dc.identifier.issn18736254
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17137
dc.language.isoEnglish
dc.subjectAdolescent
dc.subjectAdult
dc.subjectAntibodies, Helminth
dc.subjectBlotting, Western
dc.subjectEchinococcosis
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectFemale
dc.subjectHealthy Volunteers
dc.subjectHumans
dc.subjectMale
dc.subjectMass Screening
dc.subjectPrevalence
dc.subjectStudents
dc.subjectTurkey
dc.subjectUltrasonography
dc.subjectUniversities
dc.subjectYoung Adult
dc.subjectManisa
dc.subjectTurkey
dc.subjectCystic echinococcosis
dc.subjectELISA
dc.subjectEpidemiology
dc.subjectMass screening
dc.subjectUltrasonography
dc.subjectWestern blotting
dc.subjectalbendazole
dc.subjectimmunoglobulin G
dc.subjectantibody
dc.subjectdisease prevalence
dc.subjectepidemiology
dc.subjecthigher education
dc.subjectpublic health
dc.subjecttomography
dc.subjectadult
dc.subjectantibody detection
dc.subjectarticle
dc.subjectblood sampling
dc.subjectcontrolled study
dc.subjectdiagnostic procedure
dc.subjectdiagnostic test accuracy study
dc.subjectechinococcosis
dc.subjectechography
dc.subjectenzyme linked immunosorbent assay
dc.subjectfemale
dc.subjecthistopathology
dc.subjecthuman
dc.subjectintermethod comparison
dc.subjectkidney hydatid cyst
dc.subjectliver hydatid cyst
dc.subjectliver resection
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectmass screening
dc.subjectmedical history
dc.subjectprevalence
dc.subjectsensitivity and specificity
dc.subjectspleen disease
dc.subjectspleen hydatid cyst
dc.subjectTurkey (republic)
dc.subjectultrasound transducer
dc.subjectuniversity student
dc.subjectWestern blotting
dc.subjectyoung adult
dc.titleA mass screening survey of cystic echinococcosis by ultrasonography, Western blotting, and ELISA among university students in Manisa, Turkey
dc.typeArticle

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