Random/aligned electrospun PCL/PCL-collagen nanofibrous membranes: Comparison of neural differentiation of rat AdMSCs and BMSCs

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dc.contributor.authorÇapkin M.
dc.contributor.authorÇakmak S.
dc.contributor.authorKurt F.Ö.
dc.contributor.authorGumusderelioglu M.
dc.contributor.authorŞen B.H.
dc.contributor.authorTürk B.T.
dc.contributor.authorDeliloǧlu-Gürhan S.I.
dc.date.accessioned2024-07-22T08:19:44Z
dc.date.available2024-07-22T08:19:44Z
dc.date.issued2012
dc.description.abstractIn this study, the aligned (A) and randomly oriented (R) polycaprolactone (PCL-A and PCL-R) and PCL/collagen (PCL/Col-A and PCL/Col-R) nanofibers were electrospun onto smooth PCL membranes (PCLMs) prepared by solvent casting. In order to investigate the effects of chemical composition and nanotopography of fibrous surfaces on proliferation and on neural differentiation of mesenchymal stem cells (MSCs), adipose and bone marrow-derived rat MSCs (AdMSCs and BMSCs) were cultivated in suitable media i.e. inducing medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF), and cell maintenance medium (CMM). BMSCs adhered and proliferated on all nanofibrous membranes more efficiently than AdMSCs. PCL/Col-A was found as the most convenient surface supporting proliferation in both cell types. Immunofluorescence staining indicated that BMSCs and AdMSCs are prone for differentiation to oligodendrocytes more than they differentiate to other neuronal cell types. PCL-A nanofibrous membranes supported differentiation of MSCs to O4+ (an oligodendrocytes surface antigen) cells in both culture media. The intensity of immunoreactivity of O4+ cells differentiated from BMSCs on PCL-A was highest when compared with the other groups (p < 0.001). Some BIII-T signed neural cells were investigated on PCL-A nanofibrous membranes, but the intensity of immunoreactivity was lower than that of O4+ cells. In conclusion, this study can be evaluated to establish the cell therapy strategies in neurodegenerative disorders, which are relevant to oligodendrocyte abstinence using BMSCs or AdMSCs on aligned nanofibrous membranes. © 2012 IOP Publishing Ltd.
dc.identifier.DOI-ID10.1088/1748-6041/7/4/045013
dc.identifier.issn17486041
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17823
dc.language.isoEnglish
dc.publisherInstitute of Physics Publishing
dc.subjectRattus
dc.subjectCells
dc.subjectFlowcharting
dc.subjectMembranes
dc.subjectNanofibers
dc.subjectPolycaprolactone
dc.subjectRats
dc.subjectbeta1 integrin
dc.subjectCD11b antigen
dc.subjectCD45 antigen
dc.subjectcollagen
dc.subjectepidermal growth factor
dc.subjectfibroblast growth factor 2
dc.subjectintercellular adhesion molecule 1
dc.subjectmajor histocompatibility antigen class 1
dc.subjectmajor histocompatibility antigen class 2
dc.subjectnanofiber
dc.subjectpolycaprolactone
dc.subjectsolvent
dc.subjectThy 1 antigen
dc.subjectvascular cell adhesion molecule 1
dc.subjectBasic fibroblast growth factor
dc.subjectCell therapy
dc.subjectCell types
dc.subjectChemical compositions
dc.subjectCulture media
dc.subjectElectrospuns
dc.subjectEpidermal growth factors
dc.subjectImmunofluorescence staining
dc.subjectImmunoreactivities
dc.subjectMesenchymal stem cell
dc.subjectNanofibrous membranes
dc.subjectNanotopographies
dc.subjectNeural cells
dc.subjectNeural differentiations
dc.subjectNeurodegenerative disorders
dc.subjectNeuronal cell
dc.subjectOligodendrocytes
dc.subjectSolvent casting
dc.subjectSurface antigen
dc.subjectadipose derived stem cell
dc.subjectanimal cell
dc.subjectarticle
dc.subjectcell adhesion
dc.subjectcell proliferation
dc.subjectcell type
dc.subjectchemical composition
dc.subjectcontrolled study
dc.subjectculture medium
dc.subjectelectrospinning
dc.subjectglia cell
dc.subjecthematopoietic stem cell
dc.subjectimmunofluorescence microscopy
dc.subjectmembrane
dc.subjectmesenchymal stem cell
dc.subjectnerve cell differentiation
dc.subjectnonhuman
dc.subjectoligodendroglia
dc.subjectphase contrast microscopy
dc.subjectrat
dc.subjectscanning electron microscopy
dc.subjectsurface property
dc.subjecttopography
dc.subjectCell culture
dc.titleRandom/aligned electrospun PCL/PCL-collagen nanofibrous membranes: Comparison of neural differentiation of rat AdMSCs and BMSCs
dc.typeArticle

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