A comparison of two different fluorochrome stains for the detection of acid-fast bacilli in sputum specimens; [Balgam örneklerinde aside dirençli basil saptanmas? için iki farkl?ı florokrom boyan?ın karş?laşt?ır?ılmas?ı]

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dc.contributor.authorOǧuz V.A.
dc.contributor.authorSezak N.
dc.contributor.authorÖztop A.
dc.contributor.authorYapar N.
dc.contributor.authorSürücüoǧlu S.
dc.contributor.authorYüce A.
dc.date.accessioned2024-07-22T08:20:39Z
dc.date.available2024-07-22T08:20:39Z
dc.date.issued2011
dc.description.abstractAim: The early diagnosis of active tuberculosis still depends on the presence of acid-fast bacilli (AFB) in stained sputum smears. In this study, our aim was to investigate the efficiency and cost-effectiveness of two different fluorochrome stains. Materials and methods: A total of 1013 sputum specimens were collected from 642 patients. Three smears and cultures were prepared from each specimen. Double-blind and prospective laboratory procedures were performed. Slides were stained with a commercial auramine/acridine orange kit (Stain 1), an in-house preparation of auramine- rhodamine/ KMnO4 (Stain 2) and a Ziehl-Neelsen stain (EZN). Results: Of the 1013 specimens, 101 were culture positive. Among these, AFB was detected in 60 specimens by EZN, in 53 by Stain 1, in 81 by Stain 2. By cultures, the sensitivities and specificities of Stain 2 were 80.1% and 83.8%, respectively, and for Stain 1, 52.4% and 94.6% respectively. There is no significant difference between the costs of these methods. Conclusion: Stain 1 was easy to apply and inexpensive but the sensitivity of Stain 1 was lower than that of Stain 2. However, Stain 2 required longer preparation time, more work, and had a higher risk of exposure to carcinogens. In order to increase the sensitivity of Stain 1, it is suggested that the contents of the prepared Stain 1 kit could be rearranged. In tuberculosis diagnosis, this revised kit may provide practicality in use. © TÜBITAK.
dc.identifier.DOI-ID10.3906/sag-1005-797
dc.identifier.issn13000144
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18263
dc.language.isoTurkish
dc.publisherTurkiye Klinikleri
dc.rightsAll Open Access; Bronze Open Access
dc.subjectacridine
dc.subjectauramine
dc.subjectfluorochrome
dc.subjectrhodamine
dc.subjectacid fast bacterium
dc.subjectarticle
dc.subjectbacterium culture
dc.subjectbacterium detection
dc.subjectcontrolled study
dc.subjectcost effectiveness analysis
dc.subjectdiagnostic accuracy
dc.subjectdiagnostic value
dc.subjectdouble blind procedure
dc.subjectfluorochrome staining
dc.subjecthuman
dc.subjectintermethod comparison
dc.subjectpredictive value
dc.subjectprospective study
dc.subjectsensitivity and specificity
dc.subjectsputum analysis
dc.subjectsputum smear
dc.titleA comparison of two different fluorochrome stains for the detection of acid-fast bacilli in sputum specimens; [Balgam örneklerinde aside dirençli basil saptanmas? için iki farkl?ı florokrom boyan?ın karş?laşt?ır?ılmas?ı]
dc.typeArticle

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