Effect of apoptosis and response of extracellular matrix proteins after chemotherapy application on human breast cancer cell spheroids

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dc.contributor.authorOktem G.
dc.contributor.authorVatansever S.
dc.contributor.authorAyla S.
dc.contributor.authorUysal A.
dc.contributor.authorAktas S.
dc.contributor.authorKarabulut B.
dc.contributor.authorBilir A.
dc.date.accessioned2024-07-22T08:23:38Z
dc.date.available2024-07-22T08:23:38Z
dc.date.issued2006
dc.description.abstractMulticellular Tumor Spheroid (MTS) represents a three-dimentional structural form of tumors in laboratory conditions, and it has the characteristics of avascular micrometastases or intervascular spaces of big tumors. Recent studies indicate that extracellular matrix (ECM) proteins play a critical role in tumor metastasis, therefore normal and cancer cells require an ECM for survival, proliferation and differentiation. Doxorubicin and Docetaxel are widely used in the therapy of breast cancer, as well as in in vivo and in vitro studies. In this study, we examined the effect of apoptosis and proliferation of cells on the human breast cancer cell line, MCF-7, by using p53, bcl-2 and Ki67 gene expression, and the tendency to metastasis with extracellular matrix proteins, laminin and type IV collagen after chemotherapy in the spheroid model. The apoptotic cell death in situ was detected by TUNEL method. TUNEL-positive cells and positive immunoreactivities of laminin, type IV collagen, p53 and, bcl-2 were detected in the control group. There was no laminin and type IV collagen immunoreactivities in spheroids of drug groups. While TUNEL-positive cells and p53 immunoreactivity were detected in Docetaxel, Doxorubicin and Docetaxel/Doxorubicin groups, p53 immunoreactivity was not observed in the Docetaxel group. There was no bcl-2 immunoreactivity in either drug group. In addition, we did not detect Ki67 immunoreactivity in both control and drug treatment groups. However, the absence of Ki67 protein in MCF-7 breast multicellular tumor spheroids is possibly related to the cells in G0 or S phase. These chemotherapeutic agents may affect the presence of ECM proteins in this in vitro model of micrometastasis of spheroids. These findings suggest that the possible mechanism of cell death in Doxorubicin and Docetaxel/Doxorubicin treatment groups is related to apoptosis through the p53 pathway. However, we considered the possiblity that there is another control mechanism for the Docetaxel group.
dc.identifier.DOI-ID10.3892/or.15.2.335
dc.identifier.issn1021335X
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19624
dc.language.isoEnglish
dc.publisherSpandidos Publications
dc.rightsAll Open Access; Bronze Open Access
dc.subjectAntineoplastic Agents
dc.subjectApoptosis
dc.subjectCollagen Type IV
dc.subjectDoxorubicin
dc.subjectDrug Therapy, Combination
dc.subjectExtracellular Matrix Proteins
dc.subjectFemale
dc.subjectGene Expression
dc.subjectGenes, bcl-2
dc.subjectGenes, p53
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectIn Situ Nick-End Labeling
dc.subjectKi-67 Antigen
dc.subjectLaminin
dc.subjectSpheroids, Cellular
dc.subjectTaxoids
dc.subjectTumor Cells, Cultured
dc.subjectantineoplastic agent
dc.subjectcollagen type 4
dc.subjectdocetaxel
dc.subjectdoxorubicin
dc.subjectKi 67 antigen
dc.subjectlaminin
dc.subjectscleroprotein
dc.subjecttaxoid
dc.subjectapoptosis
dc.subjectarticle
dc.subjectcell culture
dc.subjectdrug combination
dc.subjectdrug effect
dc.subjectfemale
dc.subjectgene expression
dc.subjecthuman
dc.subjectimmunohistochemistry
dc.subjectmetabolism
dc.subjectmulticellular spheroid
dc.subjectnick end labeling
dc.subjectphysiology
dc.subjectproto oncogene
dc.subjecttumor suppressor gene
dc.titleEffect of apoptosis and response of extracellular matrix proteins after chemotherapy application on human breast cancer cell spheroids
dc.typeArticle

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