Significance of apoptosis related proteins on malignant transformation of ovarian tumors: A comparison between Bcl-2/Bax ratio and p53 immunoreactivity

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dc.contributor.authorZeren T.
dc.contributor.authorInan S.
dc.contributor.authorVatansever H.S.
dc.contributor.authorSayhan S.
dc.date.accessioned2024-07-22T08:15:08Z
dc.date.available2024-07-22T08:15:08Z
dc.date.issued2014
dc.description.abstractIn this study, we compared the immunoreactivities of Bcl-2, Bax and p53 proteins in ovarian tumors and related the immunohistochemical findings to the histological type of the tumors. Formalin-fixed, paraffin wax-embedded tissue sections from 40 patients who had serous-mucinous borderline tumors and serous-mucinous adenocarcinoma of the ovary (. n=. 10 each) were stained with hematoxylin-eosin (H&E). After histopathological examination, serial sections were stained immunohistochemically with primary antibodies to Bcl-2, Bax and p53 using an avidin-biotin-peroxidase method. A semi-quantitative grading system was used to compare the immunohistochemical staining intensities. The nuclear DNA fragmentation of apoptosis was determined using TUNEL method. As a result of immunohistochemical staining, increased immunoreactivity of Bcl-2 was observed in adenocarcinomas when compared to borderline tumors (. P<. 0.001). Strong immunoreactivity of Bcl-2 and mild immunoreactivities of Bax and p53 were detected in ovarian adenocarcinomas. There were no significant statistical differences in the immunoreactivity of Bax among the histological type of ovarian tumors. Whereas a balance was observed between the immunoreactivities of Bcl-2 and Bax in the borderline cases, and this balance was strongly changed toward the anti-apoptotic Bcl-2 protein in patients with adenocarcinoma. TUNEL staining of sections indicated apoptotic cells in the serous borderline tumors were about 8-fold higher than in the serous adenocarcinoma. The results of this study on apoptosis-related factors might help to develop novel protective and therapeutic approaches, such as isoflavonoids and isothiocyanates, which were associated with decreased Bcl-2/Bax ratio, against the malignant epithelial ovarian tumors. © 2014 Elsevier GmbH.
dc.identifier.DOI-ID10.1016/j.acthis.2014.07.007
dc.identifier.issn00651281
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/16686
dc.language.isoEnglish
dc.publisherElsevier GmbH
dc.subjectprotein Bax
dc.subjectprotein bcl 2
dc.subjectprotein p53
dc.subjectadult
dc.subjectapoptosis
dc.subjectArticle
dc.subjectclinical article
dc.subjectcomparative study
dc.subjectcontrolled study
dc.subjectDNA fragmentation
dc.subjectfemale
dc.subjecthistopathology
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectimmunohistochemistry
dc.subjectimmunolocalization
dc.subjectimmunoreactivity
dc.subjectmalignant transformation
dc.subjectnick end labeling
dc.subjectovary adenocarcinoma
dc.subjectovary tumor
dc.subjectquantitative analysis
dc.titleSignificance of apoptosis related proteins on malignant transformation of ovarian tumors: A comparison between Bcl-2/Bax ratio and p53 immunoreactivity
dc.typeArticle

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