Immunolocalization of integrins and fibronectin in tubal pregnancy

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dc.contributor.authorInan S.
dc.contributor.authorGiray G.
dc.contributor.authorVatansever H.S.
dc.contributor.authorOzbilgin K.
dc.contributor.authorKuscu N.K.
dc.contributor.authorSayhan S.
dc.date.accessioned2024-07-22T08:24:24Z
dc.date.available2024-07-22T08:24:24Z
dc.date.issued2004
dc.description.abstractIntegrins are a large family of cell adhesion molecules that serve as receptors involved in cell-to-cell and cell-to-matrix interactions during implantation. We studied immunohistochemical staining of integrins (α3, αV, β1, and α2β1) and fibronectin in ectopic tubal pregnancy. Thirty fallopian tube samples with ectopic pregnancies and five normal tubal segments were obtained during ligation operations; the latter specimens served as controls in the study. Formalin-fixed paraffin-embedded tissue sections were stained with hematoxylin-eosin or primary antibodies against α3, β1, αV, and α2β1 integrins and fibronectin, using the avidin-biotin-peroxidase method. A semi-quantitative grading system was used to compare staining intensities. In the control samples, immunostaining of all integrins was found in a single layer of tall columnar epithelial cells, the lamina propria (Lp) and the muscular layer. Fibronectin staining was detected in the Lp and the muscular layer. Staining intensities of α3 and β1 integrins and fibronectin were increased in the normal part of fallopian tubes with ectopic pregnancies. Staining of β1 integrin was more intense than staining of α3 and fibronectin, whereas there was no difference in αV and α2β1 integrin expression between normal tubal tissue in the ectopic pregnancy group and control tubal tissue. In the tubal pregnancy group at the site of implantation, staining intensity of α3 and β1 integrins and fibronectin was strong in decidual cells, supporting tissue and placental villi, whereas αV and α2β1 staining was mild. We concluded that integrins, especially β1 and α3, and fibronectin may play a role in progression of tubal implantation. Although the role of integrins has not yet been clearly defined, these molecules may function as markers of normal and abnormal states of receptivity. We like to suggest that integrins and fibronectin, which are needed in utero implantation, are expressed in tubal tissues during ectopic pregnancy and are involved in ectopic implantation. © 2004 Elsevier GmbH. All rights reserved.
dc.identifier.DOI-ID10.1016/j.acthis.2004.02.004
dc.identifier.issn00651281
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19964
dc.language.isoEnglish
dc.publisherElsevier GmbH
dc.subjectFallopia
dc.subjectAdhesion
dc.subjectCells
dc.subjectImmunology
dc.subjectImplants (surgical)
dc.subjectalpha3 integrin
dc.subjectalpha5 integrin
dc.subjectavidin
dc.subjectbeta1 integrin
dc.subjectbiotin
dc.subjecteosin
dc.subjectfibronectin
dc.subjectformaldehyde
dc.subjecthematoxylin
dc.subjectintegrin
dc.subjectparaffin
dc.subjectperoxidase
dc.subjectvery late activation antigen 2
dc.subjectCell adhesion molecules
dc.subjectIntegrins
dc.subjectarticle
dc.subjectcolumnar epithelium
dc.subjectcontrolled study
dc.subjectdecidua
dc.subjectectopic pregnancy
dc.subjectepithelium cell
dc.subjectfemale
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectimmunohistochemistry
dc.subjectimmunolocalization
dc.subjectnidation
dc.subjectprotein expression
dc.subjectuterine tube
dc.subjectuterine tube pregnancy
dc.subjectProteins
dc.titleImmunolocalization of integrins and fibronectin in tubal pregnancy
dc.typeArticle

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