Effect of insulin on rat ovarian leptin expression by immunohistochemical staining

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dc.contributor.authorKuscu N.K.
dc.contributor.authorOzbilgin K.
dc.contributor.authorInan S.
dc.contributor.authorGiray G.
dc.contributor.authorCeylan E.
dc.contributor.authorKoyuncu F.
dc.date.accessioned2024-07-22T08:24:49Z
dc.date.available2024-07-22T08:24:49Z
dc.date.issued2003
dc.description.abstractAim: Leptin and insulin may interact in regulating ovarian steroid synthesis. The objective of this study was to investigate immunohistochemical staining of leptin in normal rat ovarian tissues and in rats treated with insulin and insulin plus human chorinoic gonadotropin (hCG). Methods: Paraffin blocks of rat ovarian tissues from a previous study, in which 18 adult, female Wistar rats with an average weight of 250 g were divided into three groups to receive either saline solution, human insulin (2 U/day) or human insulin (2 U/day) plus hCG (4 U/day) for 4 weeks, were used in this study to compare the effects on leptin staining. The results were analysed using a semiquantitative scoring system, such as mild, moderate and strong. Results: No staining was observed in granulosa cells and theca interna cells of normal ovarian tissues. Theca externa cells had mild staining intensity (+), corpus luteum had moderate (+ +) and stroma had mild (+) staining intensity. Histological structure was impaired in the insulin group, luteinized cells had-mild staining, there was no difference in other cell groups. Only theca externa cells of the developing follicles were stained in insulin plus hCG group, luteinized cells again had mild staining. Conclusions: Besides damaging the rat ovarian structure, insulin reduced staining intensity of leptin in luteinized cells. Insulin may stimulate ovarian steroid synthesis not only through its own receptors, but also by acting on the leptin expression of these cells.
dc.identifier.DOI-ID10.1111/j.1341-8076.2003.00140.x
dc.identifier.issn13418076
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/20143
dc.language.isoEnglish
dc.subjectAnimals
dc.subjectChorionic Gonadotropin
dc.subjectDrug Administration Schedule
dc.subjectFemale
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectInsulin
dc.subjectLeptin
dc.subjectOvarian Follicle
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectStaining and Labeling
dc.subjectchorionic gonadotropin
dc.subjecthuman insulin
dc.subjectinsulin
dc.subjectleptin
dc.subjectsodium chloride
dc.subjectsteroid
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectcomparative study
dc.subjectcontrolled study
dc.subjectcorpus luteum
dc.subjectdrug effect
dc.subjectfemale
dc.subjectgranulosa cell
dc.subjecthistology
dc.subjectimmunohistochemistry
dc.subjectluteal cell
dc.subjectnonhuman
dc.subjectovary
dc.subjectprotein expression
dc.subjectquantitative analysis
dc.subjectrat
dc.subjectrat strain
dc.subjectscoring system
dc.subjectsteroidogenesis
dc.subjectstroma
dc.subjecttheca cell
dc.subjecttissue structure
dc.titleEffect of insulin on rat ovarian leptin expression by immunohistochemical staining
dc.typeArticle

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