13-93B3 Bioactive Glass: A New Scaffold for Transplantation of Stem Cell-Derived Chondrocytes

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dc.contributor.authorGunhan K.
dc.contributor.authorBariskan S.
dc.contributor.authorUz U.
dc.contributor.authorVatansever S.
dc.contributor.authorKivanc M.
dc.date.accessioned2024-07-22T08:10:20Z
dc.date.available2024-07-22T08:10:20Z
dc.date.issued2018
dc.description.abstractResearch using animal models gives human trials hope for recovery in many fields of regenerative medicine, although they are sometimes poor predictors for human experiences. Our goal was to investigate whether rat chondrocytes, differentiated from adipose-derived stem cells, could be transplanted using a new, easily shaped, bioactive glass scaffold, and to show the immunohistochemical results. Intraperitoneal and retroperitoneal adipose tissue was extracted from 6 male Wistar albino type rats. The fatty tissue samples were fragmented and incubated. Chondrogenic differentiation was carried out and collagen type II, bFGF, and Sox-9 immunohistochemical characterization analysis was performed. Differentiated chondrocytes were implanted on 13-93B3 bioactive glass scaffolds and transplanted into the right ears of the rats. As control, only the biomaterial was transplanted into the left ears of the rats. After 1 month, the rats were sacrificed and transplantation areas were examined immunohistochemically. Histological examination of control samples from the left ears revealed that the biomaterial was covered with connective tissue, its general structure was preserved, and resorption of the scaffold had started. In specimens from the right ears, the biomaterial was covered with connective tissue, its structure was preserved, cartilage cells were present around the biomaterial, and the presence of cartilage tissue was demonstrated immunohistochemically. In conclusion, 13-93B3 bioactive glass scaffold contributed to the formation of new collagen and the survival of chondrocytes, and is a promising new biomaterial that will prove very useful in regenerative medicine. © Copyright 2017 by Mutaz B. Habal, MD.
dc.identifier.DOI-ID10.1097/SCS.0000000000004037
dc.identifier.issn10492275
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15178
dc.language.isoEnglish
dc.publisherLippincott Williams and Wilkins
dc.subjectAdipose Tissue
dc.subjectAnimals
dc.subjectBiocompatible Materials
dc.subjectCartilage
dc.subjectCell Differentiation
dc.subjectChondrocytes
dc.subjectChondrogenesis
dc.subjectCollagen Type II
dc.subjectFibroblast Growth Factor 2
dc.subjectMale
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectStem Cells
dc.subjectTissue Scaffolds
dc.subjectbiomaterial
dc.subjectcollagen type 2
dc.subjectfibroblast growth factor 2
dc.subjectadipose tissue
dc.subjectanimal
dc.subjectcartilage
dc.subjectcell differentiation
dc.subjectchemistry
dc.subjectchondrocyte
dc.subjectchondrogenesis
dc.subjectcytology
dc.subjectgrowth, development and aging
dc.subjectmale
dc.subjectmetabolism
dc.subjectrat
dc.subjectstem cell
dc.subjecttissue scaffold
dc.subjecttransplantation
dc.subjectWistar rat
dc.title13-93B3 Bioactive Glass: A New Scaffold for Transplantation of Stem Cell-Derived Chondrocytes
dc.typeArticle

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