Molecular typing and sequencing of adenovirus isolated from a conjunctivitis outbreak in a neonatal intensive care unit by PCR

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dc.contributor.authorÇiçek C.
dc.contributor.authorŞanlidaǧ T.
dc.contributor.authorSiyah Bilgin B.
dc.contributor.authorPullukçu H.
dc.contributor.authorAkçali S.
dc.contributor.authorAltun Köroǧlu O.
dc.contributor.authorYalaz M.
dc.contributor.authorKültürsay N.
dc.date.accessioned2024-07-22T08:19:03Z
dc.date.available2024-07-22T08:19:03Z
dc.date.issued2012
dc.description.abstractAim: We aimed to evaluate the molecular typing of adenovirus isolated during an epidemic at the Ege University Children's Hospital neonatal intensive care unit (NICU). Materials and methods: During the NICU outbreak management, 40 clinical samples (from 15 newborn infants and 25 health care providers) were sent to a microbiology laboratory in viral transport media. All the samples were processed using a direct fluorescent antibody (DFA) test and a shell vial cell culture followed by adenovirus polymerase chain reaction (PCR) and DNA sequencing. PCR and DNA sequencing for adenovirus hexon gene hypervariable regions 1-6 were done after DNA extraction from clinical specimens. Adenovirus typing was done using BLAST analysis. Results: Ten adenoviruses were isolated from 4 out of 10 infants, 3 out of 5 hospital staff with conjunctivitis, and 3 asymptomatic staff. Ten positive samples were identified as adenovirus type 8 by using BLAST analysis. Conclusion: We isolated adenovirus type 8, one of the most common serotypes causing conjunctivitis, during an adenovirus outbreak in our NICU. The highest positivity was obtained using the PCR method. Although DFA was positive in a limited number of cases, this test was applied rapidly at the beginning of the epidemic and contributed to the prevention of further spread. © TÜBİTAK.
dc.identifier.DOI-ID10.3906/sag-1206-104
dc.identifier.issn13000144
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17491
dc.language.isoEnglish
dc.rightsAll Open Access; Bronze Open Access
dc.subjectvirus DNA
dc.subjectAdenovirus
dc.subjectadenovirus 8
dc.subjectarticle
dc.subjectclinical article
dc.subjectclinical laboratory
dc.subjectconjunctivitis
dc.subjectculture medium
dc.subjectDNA sequence
dc.subjectepidemic
dc.subjectfemale
dc.subjectfluorescent antibody technique
dc.subjectHexon gene
dc.subjecthospital personnel
dc.subjecthuman
dc.subjectinfant
dc.subjectmale
dc.subjectmolecular typing
dc.subjectnewborn
dc.subjectnewborn intensive care
dc.subjectnonhuman
dc.subjectpediatric hospital
dc.subjectpolymerase chain reaction
dc.subjectsequence analysis
dc.subjectthroat culture
dc.subjectvirus culture
dc.subjectvirus gene
dc.subjectvirus identification
dc.subjectvirus isolation
dc.subjectvirus typing
dc.titleMolecular typing and sequencing of adenovirus isolated from a conjunctivitis outbreak in a neonatal intensive care unit by PCR
dc.typeArticle

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