Molecular Characterization of Paederus Spp (Coleoptera: Staphylinidae, Paederinae) the Agent of Human Linear Dermatitis in the Caspian Sea Coast, North of Iran

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dc.contributor.authorHeydari A.
dc.contributor.authorAnlaş S.
dc.contributor.authorBakhshi H.
dc.contributor.authorKoosha M.
dc.contributor.authorChoubdar N.
dc.contributor.authorPanahi-Moghadam S.
dc.contributor.authorOshaghi M.A.
dc.date.accessioned2024-07-22T08:03:05Z
dc.date.available2024-07-22T08:03:05Z
dc.date.issued2023
dc.description.abstractBackground: A combined morphological and molecular survey was performed to determine the agent of human linear dermatitis Paederus Fabricius, 1775 (Coleoptera: Staphylinidae, Paederinae) species composition in Mazandaran Province in the Caspian Sea coast in northern Iran, where most of linear dermatitis cases of the country occurred. Methods: Altogether, 397 Paederus specimens were collected from May to August 2021 and classified using morphological characters and ITS2-rDNA sequence analysis. Results: Morphological investigation revealed that all the specimens were Paederus fuscipes. ITS2 polymerase chain reaction (PCR) direct-sequences and the profiles of restriction fragment length polymorphism (RFLP) derived from digestion of PCR products by HinfI, HpaII, and SalI enzymes were identical confirming the morphological results, implying that all specimens belonged to a single taxon. Conclusion: Paederus fuscipes (Fabricius, 1775) is considered the dominant taxon and responsible for linear dermatitis in Mazandaran Province. To our knowledge, we have provided the first molecular typing of Paederus beetles at the species level, suggesting that ITS2-rDNA characterization is an alternative tool for species discrimination of Paederus spp. Copyright © 2023 The Authors. Published by Tehran University of Medical Sciences.
dc.identifier.DOI-ID10.18502/jad.v17i1.13206
dc.identifier.issn23221984
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12125
dc.language.isoEnglish
dc.publisherTehran University of Medical Sciences
dc.rightsAll Open Access; Gold Open Access; Green Open Access
dc.subjectenzyme
dc.subjectHinfI
dc.subjectHpaII
dc.subjectinternal transcribed spacer
dc.subjectSalI
dc.subjecttype II site specific deoxyribonuclease
dc.subjectunclassified drug
dc.subjectadult
dc.subjectanimal experiment
dc.subjectArticle
dc.subjectbeetle
dc.subjectCaspian Sea
dc.subjectdigestion
dc.subjectDNA extraction
dc.subjectDNA sequence
dc.subjectGenBank
dc.subjectgene amplification
dc.subjectgeographic distribution
dc.subjectIran
dc.subjectmale
dc.subjectmolecular fingerprinting
dc.subjectmolecular typing
dc.subjectmorphology
dc.subjectnonhuman
dc.subjectPaederus dermatitis
dc.subjectpolymerase chain reaction
dc.subjectrestriction fragment length polymorphism
dc.subjectScarabaeidae
dc.subjectseashore
dc.subjectsequence alignment
dc.subjectsequence analysis
dc.subjectspecies composition
dc.subjectspecies identification
dc.subjectStaphylinidae
dc.titleMolecular Characterization of Paederus Spp (Coleoptera: Staphylinidae, Paederinae) the Agent of Human Linear Dermatitis in the Caspian Sea Coast, North of Iran
dc.typeArticle

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