In-vitro evaluation of effects of mesenchymal stem cells on tlr3, tlr7/8 and tlr9-activated natural killer cells

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dc.contributor.authorOzdemir A.T.
dc.contributor.authorKirmaz C.
dc.contributor.authorOzdemir R.B.O.
dc.contributor.authorDegirmenci P.
dc.contributor.authorOztatlici M.
dc.contributor.authorDegirmenci M.
dc.date.accessioned2024-07-22T08:06:27Z
dc.date.available2024-07-22T08:06:27Z
dc.date.issued2021
dc.description.abstractObjectives: In this study, it was aimed to investigate the immunomodulatory effects of Mesenchymal stem cells (MSCs) on Natural Killer (NK) cells activated by Toll-like receptor (TLR) agonists. Methods: MDA-MB-231, MCF-7 and NK-92 cells were induced with TLR3, TLR7/8 and TLR9 agonists and co-cultured with MSCs. Alterations in IFN-γ, TNF-α, Granzyme-b and Perforin expressions were determined by qPCR method, CD69 and CD107a expressions were determined by flow cytometry, and cytotoxicity was determined by MTT-assay. Results: All TLR agonists significantly increased the expressions of the IFN-γ, TNF-α, Granzyme-b, Perforin, CD69 and CD107a in-vitro. We determined that the cytokine, cytotoxic molecules, and activation markers of NK-92 cells interact-ing with breast tumor cells significantly increased by TLR3 and TLR9 agonists. However, suppression rather than activation occurred on the NK-92 cells due to the simultaneous induction of the immunosuppressive effects of MSCs by these agonists. On the other hand, the TLR7/8 agonists provided a low NK-92 induction, however, the inhibitory effects of MSCs were not triggered. Therefore, it provided a more significant activation than TLR3 and TLR9 agonists. Conclusion: Our findings suggested that TLR7/8 agonists may be a better choice to induce antitumor effects of NK cells in a tumor tissue rich in MSCs. © 2021 by Eurasian Journal of Medicine and Oncology.
dc.identifier.DOI-ID10.14744/ejmo.2021.15684
dc.identifier.issn25872400
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13535
dc.language.isoEnglish
dc.publisherKare Publishing
dc.rightsAll Open Access; Gold Open Access
dc.subjectCD69 antigen
dc.subjectgamma interferon
dc.subjectgranzyme B
dc.subjectlysosome associated membrane protein 1
dc.subjectperforin
dc.subjecttoll like receptor 3
dc.subjecttoll like receptor 7
dc.subjecttoll like receptor 8
dc.subjecttoll like receptor 9
dc.subjecttumor necrosis factor
dc.subjectArticle
dc.subjectcell activation
dc.subjectcell function
dc.subjectcell interaction
dc.subjectcoculture
dc.subjectcontrolled study
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman tissue
dc.subjectimmunomodulation
dc.subjectimmunosuppressive treatment
dc.subjectin vitro study
dc.subjectMCF-7 cell line
dc.subjectMDA-MB-231 cell line
dc.subjectmesenchymal stem cell
dc.subjectnatural killer cell
dc.subjectNK-92 cell line
dc.subjectprotein expression
dc.subjectreal time polymerase chain reaction
dc.subjecttumor immunity
dc.titleIn-vitro evaluation of effects of mesenchymal stem cells on tlr3, tlr7/8 and tlr9-activated natural killer cells
dc.typeArticle

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