The cytotoxic and antiproliferative effect of Polygala saponin XLIV on the human colorectal carcinoma cell line
| dc.contributor.author | Becer, E | |
| dc.contributor.author | Hanoglu, A | |
| dc.contributor.author | Ünlü, A | |
| dc.contributor.author | Aydin, ZU | |
| dc.contributor.author | Dönmez, AA | |
| dc.contributor.author | Jurt, S | |
| dc.contributor.author | Vatansever, SH | |
| dc.contributor.author | Çalis, I | |
| dc.date.accessioned | 2025-04-10T10:36:39Z | |
| dc.date.available | 2025-04-10T10:36:39Z | |
| dc.description.abstract | Objectives Saponins are secondary metabolites naturally found in plants with diverse pharmacological properties such as anticancer. This research aimed to explore the anti-cancer properties of Polygalasaponin XLIV (PS-XLIV) in a human colorectal carcinoma cell line derived from Polygala vulgaris roots.Methods HCT166 cells were treated with different PS-XLIV concentrations and incubated for 24 and 48 h. We used immunocytochemistry to investigate PS-XLIV's anti-cancer properties, employing antibodies targeting WNT3A, WNT11, STAT3, beta-catenin, and Ki-67. The IC50 value of PS-XLIV was 80 mu g/mL in HCT116 cells. WNT11, STAT3, beta-catenin, and Ki-67. Immunoreactivities significantly decreased in PS-XLIV-treated HCT116 cells than in control group cells.Results After PS-XLIV treatment, the epithelial morphology of cells was protected; however, the number of cells was less than that of the control group cells. While WNT3A immunoreactivity was similar in both groups, WNT11 and beta-catenin immunoreactivities were decreased after PS-XLIV application. In addition, the PS-XLIV treated group exhibited significantly weaker Ki-67 immunoreactivity, STAT3 immunoreactivty was moderated after PS-XLIV application.Conclusions For the first time, the anticancer effects of PS-XLIV isolated from P. vulgaris on HCT116 cells were shown. The anticancer effect may involve PS-XLIV reducing WNT11, beta-catenin, STAT3, and Ki-67 activation pathways in HCT116 cells. | |
| dc.identifier.e-issn | 1303-829X | |
| dc.identifier.issn | 0250-4685 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14701/42465 | |
| dc.language.iso | English | |
| dc.title | The cytotoxic and antiproliferative effect of Polygala saponin XLIV on the human colorectal carcinoma cell line | |
| dc.type | Article; Early Access |