The effects of DBcAMP on the expression of eNOS, iNOS and TGF-beta in rat heart tissue

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dc.contributor.authorEkerbicer N.
dc.contributor.authorInan S.
dc.contributor.authorTarakci F.
dc.contributor.authorZeren T.
dc.date.accessioned2024-07-22T08:21:59Z
dc.date.available2024-07-22T08:21:59Z
dc.date.issued2009
dc.description.abstractDibutryl (DB) adenosine 3′,5′-cyclic monophosphate (cAMP) is an important modulator of physiological functions. To determine the protective effects of DBcAMP on heart tissue, we evaluated changes in immunoreactivity of endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS) and transforming growth factor-beta (TGF-β) in left cervical vagotomized rats treated with DBcAMP. Male rats were divided into four groups. In Group 1, animals were subjected to a left cervical vagotomy. Group 2 received a 1 ml bolus injection of 15 ml/kg DBcAMP in addition to the left vagotomy. DBcAMP alone was given to Group 3 and Group 4 was the control group. For each animal, mean arterial pressure (MAP) and heart rate (HR) were measured. For indirect immunohistochemistry, anti-eNOS, anti-iNOS, and anti-TGF-β primary antibodies were used. In Group 1, MAP and HR values decreased slightly. In Groups 2 and 3, DBcAMP induced a statistically significant drop in HR and MAP. In Group 1, strong eNOS, iNOS, and TGF-β immunoreactivities were observed. Immunostaining intensities decreased in Groups 2 and 3. The results of the study reported here suggest that increased immunoreactivities of eNOS, iNOS, and TGF-β might contribute to the effects on the heart tissue after left vagotomy and imply that DBcAMP acts on heart tissue via nitric oxide.
dc.identifier.DOI-ID10.1080/10520290902752408
dc.identifier.issn14737760
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18871
dc.language.isoEnglish
dc.subjectAnimals
dc.subjectBucladesine
dc.subjectDose-Response Relationship, Drug
dc.subjectGene Expression
dc.subjectMale
dc.subjectMyocardium
dc.subjectNitric Oxide Synthase Type III
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectSignal Transduction
dc.subjectTransforming Growth Factor beta
dc.subjectAnimalia
dc.subjectRattus
dc.titleThe effects of DBcAMP on the expression of eNOS, iNOS and TGF-beta in rat heart tissue
dc.typeArticle

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