Epidemiological analysis of Leishmania tropica strains and giemsa-stained smears from Syrian and Turkish leishmaniasis patients using multilocus microsatellite typing (MLMT)

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dc.contributor.authorKarakuş M.
dc.contributor.authorNasereddin A.
dc.contributor.authorOnay H.
dc.contributor.authorKaraca E.
dc.contributor.authorÖzkeklikçi A.
dc.contributor.authorJaffe C.L.
dc.contributor.authorKuhls K.
dc.contributor.authorÖzbilgin A.
dc.contributor.authorErtabaklar H.
dc.contributor.authorDemir S.
dc.contributor.authorÖzbel Y.
dc.contributor.authorTöz S.
dc.date.accessioned2024-07-22T08:10:50Z
dc.date.available2024-07-22T08:10:50Z
dc.date.issued2017
dc.description.abstractTurkey is located in an important geographical location, in terms of the epidemiology of vector-borne diseases, linking Asia and Europe. Cutaneous leishmaniasis (CL) is one of the endemic diseases in a Turkey and according to the Ministry Health of Turkey, 45% of CL patients originate from Şanlıurfa province located in southeastern Turkey. Herein, the epidemiological status of CL, caused by L. tropica, in Turkey was examined using multilocus microsatellite typing (MLMT) of strains obtained from Turkish and Syrian patients. A total of 38 cryopreserved strains and 20 Giemsa-stained smears were included in the present study. MLMT was performed using 12 highly specific microsatellite markers. Delta K (ΔK) calculation and Bayesian statistics were used to determine the population structure. Three main populations (POP A, B and C) were identified and further examination revealed the presence of three subpopulations for POP B and C. Combined analysis was performed using the data of previously typed L. tropica strains and Mediterranean and Şanlıurfa populations were identified. This finding suggests that the epidemiological status of L. tropica is more complicated than expected when compared to previous studies. A new population, comprised of Syrian L. tropica samples, was reported for the first time in Turkey, and the data presented here will provide new epidemiological information for further studies. © 2017 Karakuş et al.
dc.identifier.DOI-ID10.1371/journal.pntd.0005538
dc.identifier.issn19352727
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15404
dc.language.isoEnglish
dc.publisherPublic Library of Science
dc.rightsAll Open Access; Gold Open Access; Green Open Access
dc.subjectAzure Stains
dc.subjectBayes Theorem
dc.subjectDNA, Protozoan
dc.subjectGenetic Variation
dc.subjectGeographic Mapping
dc.subjectHumans
dc.subjectLeishmania tropica
dc.subjectLeishmaniasis, Cutaneous
dc.subjectMicrosatellite Repeats
dc.subjectMultilocus Sequence Typing
dc.subjectPhylogeny
dc.subjectSyria
dc.subjectTurkey
dc.subjectmicrosatellite DNA
dc.subjectGiemsa stain
dc.subjectprotozoal DNA
dc.subjectArticle
dc.subjectDNA extraction
dc.subjectgeographic distribution
dc.subjectGiemsa stain
dc.subjecthuman
dc.subjectLeishmania tropica
dc.subjectmigration
dc.subjectmulticenter study
dc.subjectmultilocus sequence typing
dc.subjectneighbor joining method
dc.subjectnonhuman
dc.subjectphylogeny
dc.subjectreal time polymerase chain reaction
dc.subjectskin leishmaniasis
dc.subjectSyrian
dc.subjectTurk (people)
dc.subjectTurkey (republic)
dc.subjectBayes theorem
dc.subjectclassification
dc.subjectgenetic variation
dc.subjectgeographic mapping
dc.subjectGiemsa stain
dc.subjectisolation and purification
dc.subjectLeishmaniasis, Cutaneous
dc.subjectmultilocus sequence typing
dc.subjectSyrian Arab Republic
dc.subjectTurkey
dc.titleEpidemiological analysis of Leishmania tropica strains and giemsa-stained smears from Syrian and Turkish leishmaniasis patients using multilocus microsatellite typing (MLMT)
dc.typeArticle

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