Isolation, characterization and numerical taxonomy of novel oxalate-oxidizing bacteria

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dc.contributor.authorSahin N.
dc.contributor.authorGökler I.
dc.contributor.authorTamer A.Ü.
dc.date.accessioned2024-07-22T08:25:17Z
dc.date.available2024-07-22T08:25:17Z
dc.date.issued2002
dc.description.abstractThe present work is aimed at providing additional new pure cultures of oxalate utilizing bacteria and its preliminary characterization for further work in the field of oxalate-metabolism and taxonomic studies. The taxonomy of 14 mesophilic, aerobic oxalotrophic bacteria isolated by an enrichment culture technique from soil, rhizospheres, and the juice of the petiole/stem tissue of plants was investigated. Isolates were characterized with 95 morphological, biochemical and physiological tests. Cellular lipid components and carotenoids of isolates were also studied as an aid to taxonomic characterization. All isolates were Gram-negative, oxidase and catalase positive and no growth factors were required. In addition to oxalates, some of the strains grow on methanol and/or formate. The taxonomic similarities among isolates, reference strains or previously reported oxalotrophic bacteria were analysed by using the Simple Matching (SSM) and Jaccard (SJ) Coefficients. Clustering was performed by using the unweighted pair group method with arithmetic averages (UPGMA) algorithm. The oxalotrophic strains formed five major and two single-member clusters at the 70-86% similarity level. Based on the numerical taxonomy, isolates were separated into three phenotypic groups. Pink-pigmented strains belonged to Methylobacterium extorquens, yellow-pigmented strains were most similar to Pseudomonas sp. YOx and Xanthobacter autotrophicus, and heterogeneous non-pigmented strains were closely related to genera Azospirillum, Ancylobacter, Burkholderia and Pseudomonas. New strains belonged to the genera Pseudomonas, Azospirillum and Ancylobacter that differ taxonomically from other known oxalate oxidizers were obtained. Numerical analysis indicated that some strains of the yellow-pigmented and nonpigmented clusters might represent new species.
dc.identifier.issn12258873
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/20355
dc.language.isoEnglish
dc.subjectAncylobacter
dc.subjectAzospirillum
dc.subjectBacteria (microorganisms)
dc.subjectBurkholderia
dc.subjectMethylobacterium
dc.subjectMethylobacterium extorquens
dc.subjectNegibacteria
dc.subjectPseudomonas
dc.subjectPseudomonas sp.
dc.subjectXanthobacter
dc.subjectXanthobacter autotrophicus
dc.subjectcarotenoid
dc.subjectcatalase
dc.subjectformic acid
dc.subjectgrowth factor
dc.subjectmethanol
dc.subjectoxalic acid
dc.subjectoxidoreductase
dc.subjectpigment
dc.subjectalgorithm
dc.subjectancyclobacter
dc.subjectarticle
dc.subjectAzospirillum
dc.subjectbacterium culture
dc.subjectbacterium identification
dc.subjectbacterium isolation
dc.subjectbiochemistry
dc.subjectBurkholderia
dc.subjectcontrolled study
dc.subjectGram negative bacterium
dc.subjectmathematical computing
dc.subjectMethylobacterium extorquens
dc.subjectmorphology
dc.subjectnonhuman
dc.subjectpetiole
dc.subjectphenotype
dc.subjectphysiology
dc.subjectplant stem
dc.subjectPseudomonas
dc.subjectrhizosphere
dc.subjectsoil
dc.subjecttaxonomy
dc.subjectXanthobacter
dc.subjectxanthobacter autotrophicus
dc.titleIsolation, characterization and numerical taxonomy of novel oxalate-oxidizing bacteria
dc.typeArticle

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