The maturity of intestinal neomucosa: Integrin expression and ultrastructural aspects

Simple item page
dc.contributor.authorGünşar C.
dc.contributor.authorVatansever H.S.
dc.contributor.authorArslan O.A.
dc.contributor.authorŞencan A.
dc.contributor.authorMüftüoǧlu S.
dc.contributor.authorÖzbilgin K.
dc.contributor.authorKaymaz F.
dc.contributor.authorMir E.
dc.date.accessioned2024-07-22T08:24:18Z
dc.date.available2024-07-22T08:24:18Z
dc.date.issued2004
dc.description.abstractBackground/purpose The maturity of neomucosa growing on a serosal surface for the treatment of short bowel syndrome still is questionable. The aim of this study was to evaluate the intestinal neomucosa to assess its histologic maturity. Methods A 6-cm-long isolated ileal segment (IS) was prepared in 8 Wistar albino-type rats. The IS was divided from the antimesenteric side, and 2 intestinal tubes were established, which shared a common wall and a common pedicle. After ileal biopsy sampling for the control group (CG), the IS was fashioned into a mucous fistula. Eight weeks later, all the rats were killed, and the ISs were investigated for neomucosal growth. Sections were prepared with periodic acid shift (PAS) and H & E staining for light microscopy. They also were evaluated by transmission electron microscopy. The microscopic morphology of the 2 groups was evaluated. Immunohistochemical staining was performed to show the expression of the tissue β1, α3 and α2β1 integrin subunits of both the neomucosa (NS) and control group (CG) segments. Results Sections of the NS showed a well-arranged columnar epithelial cell layer with goblet cells that were generally located superficially and with a complete basement membrane. Under the electron microscope, the sections from the NS group showed an epithelial cell layer with proper microvilli of the same height, although they were shorter than those of the CG, and tight intercellular junctions between the epithelial cells. Significant differences between the NS and CG groups were found in the measurements of villus width at base, microvillus surface, and microvillus height. The lamina propria consisted of rich collagen fibers and active fibroblasts in the NS group. In the immunohistochemical staining, although β1 integrine showed a dense distribution (+++) in the lamina propria, particularly localizing at the depth of the tunica mucosa layer, α3 integrin was observed to have a less dense immunoreactivity (++) in both groups. The expression of α2β1 integrin showed slight and dispersed (+) staining. Conclusions The NS showed histologic maturity and ultimate structural similarity with the native small bowel mucosa, which provides strong indirect evidence for the proper functioning of the neomucosa. © 2004 Elsevier Inc. All rights reserved.
dc.identifier.DOI-ID10.1016/j.jpedsurg.2004.05.008
dc.identifier.issn00223468
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19921
dc.language.isoEnglish
dc.subjectAnastomosis, Surgical
dc.subjectAnimals
dc.subjectAntigens, CD29
dc.subjectBiopsy
dc.subjectEpithelial Cells
dc.subjectIleum
dc.subjectIntegrin alpha2beta1
dc.subjectIntegrin alpha3
dc.subjectIntestinal Mucosa
dc.subjectMicroscopy, Electron
dc.subjectMicrovilli
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectShort Bowel Syndrome
dc.subjectStaining and Labeling
dc.subjectSurgically-Created Structures
dc.subjectWound Healing
dc.subjectalpha3 integrin
dc.subjectbeta1 integrin
dc.subjectcollagen fiber
dc.subjecteosin
dc.subjecthematoxylin
dc.subjectprotein subunit
dc.subjectvery late activation antigen 2
dc.subjectalbinism
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectbasement membrane
dc.subjectcell activation
dc.subjectcell junction
dc.subjectcell surface
dc.subjectcellular distribution
dc.subjectcolumnar epithelium
dc.subjectcontrolled study
dc.subjectdispersion
dc.subjectevaluation
dc.subjectfibroblast
dc.subjectgoblet cell
dc.subjectheight
dc.subjecthistology
dc.subjectileum
dc.subjectimmunohistochemistry
dc.subjectimmunoreactivity
dc.subjectintestine fistula
dc.subjectlamina propria
dc.subjectmesentery
dc.subjectmicromorphology
dc.subjectmicrovillus
dc.subjectnonhuman
dc.subjectobservation
dc.subjectperiodic acid Schiff stain
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectprotein localization
dc.subjectprotein structure
dc.subjectrat
dc.subjectsmall intestine mucosa
dc.subjecttissue growth
dc.subjecttransmission electron microscopy
dc.subjectultrastructure
dc.titleThe maturity of intestinal neomucosa: Integrin expression and ultrastructural aspects
dc.typeArticle

Files

Collections

Scopus Koleksiyonu