Apoptosis seems to be the major process while surface and neural ectodermal layers detach during neurulation

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dc.contributor.authorSelçuki M.
dc.contributor.authorVatansever S.
dc.contributor.authorUmur A.S.
dc.contributor.authorTemiz C.
dc.contributor.authorSayin M.
dc.date.accessioned2024-07-22T08:22:16Z
dc.date.available2024-07-22T08:22:16Z
dc.date.issued2008
dc.description.abstractObjective: The aim of this study is to demonstrate the process of detaching neural and surface ectodermal layers soon after the neurulation completes. Materials and methods: Specific pathogen-free chicken egg embryos were used to investigate the neurulation procedure. Ten eggs were saved as controls. The other ten eggs were opened at the 30th hour of embryo development and cultured with Z-VAD-FMK (peptide caspase inhibitor) to investigate the results of the apoptosis inhibition. Embryos were placed and developed up to 48 h in the culture medium. To detect apoptotic cells between neural and surface dermal layers, immunoreactivity of p53 and terminal uridine deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were used. Results: While the control group shows positive immunoreactivity of p53 and TUNEL-positive apoptotic cells at the site where the neural folds detach from the surface ectoderm, no TUNEL activity and no detachment were detected in the apoptosis-inhibited group. Conclusion: As inhibition of apoptosis prevented the detachment of the neural and surface ectodermal layers from each other at the end of the neurulation, inhibition of apoptosis seemed to cause a considerable embryological error accounted for congenital dermal sinus tractus maldevelopment. © Springer-Verlag 2007.
dc.identifier.DOI-ID10.1007/s00381-007-0527-y
dc.identifier.issn02567040
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18999
dc.language.isoEnglish
dc.subjectAmino Acid Chloromethyl Ketones
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectChick Embryo
dc.subjectCysteine Proteinase Inhibitors
dc.subjectEctoderm
dc.subjectEmbryonic Development
dc.subjectGene Expression Regulation, Developmental
dc.subjectIn Situ Nick-End Labeling
dc.subjectNeurons
dc.subjectTumor Suppressor Protein p53
dc.subjectbenzyloxycarbonylvalylalanylaspartyl fluoromethyl ketone
dc.subjectprotein p53
dc.subjectanimal tissue
dc.subjectapoptosis
dc.subjectarticle
dc.subjectchick embryo
dc.subjectcontrolled study
dc.subjectculture medium
dc.subjectectoderm
dc.subjectembryo
dc.subjectembryo culture
dc.subjectembryo development
dc.subjectimmunoreactivity
dc.subjectnervous system development
dc.subjectneurulation
dc.subjectnick end labeling
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectyolk sac
dc.titleApoptosis seems to be the major process while surface and neural ectodermal layers detach during neurulation
dc.typeArticle

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