Evaluation of scale-up parameters of bioethanol production from Escherichia coli KO11; [Escherichia coli KO11 suşundan biyoetanol üretimi için ölçek büyütme parametrelerinin değerlendirilmesi]
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Date
2015
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Abstract
into fuel ethanol, as one of the cleanest liquid fuel alternatives to fossil fuels. However, industrial production of bioethanol is related with successful scaling-up studies. Methods: In this study, the experimental designs of scale-up procedures based on constant mixing time, impeller tip speed and oxygen mass transfer coefficient were performed in 8 L stirred tank reactor and were compared in terms of product yield and productivity with those obtained from 2 L stirred tank reactor using quince pomace as a substrate for bioethanol production by Escherichia coli KO11. Results: Scale-up based on constant mixing time yielded a maximum ethanol concentration of 23.42 g/L which corresponded to 0.4 g ethanol/ g reduced sugar in 8 L stirred tank reactor. Moreover, shear stress increased only 1.1 fold which resulted in low cell damage and high cell viability. Conclusion: Constant mixing time was identified as the most important key parameter especially for scaling-up of viscous fermentation broths of bioethanol production due to the significance of the homogeneity. © 2015, Turkish Biochemistry Society. All rights reserved.
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Cydonia oblonga , Escherichia coli , alcohol , alcohol production , Article , biomass , cell viability , chemical parameters , density , enzyme substrate , Escherichia coli , fermentation , mathematical parameters , nonhuman , oxygen mass transfer coefficient , process optimization , shear stress , viscosity