Effects of caffeic acid phenethyl ester on matrix molecules and angiogenetic and anti-angiogenetic factors in gastric cancer cells cultured on different substrates

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dc.contributor.authorKosova F.
dc.contributor.authorKurt F.O.
dc.contributor.authorOlmez E.
dc.contributor.authorTuʇlu I.
dc.contributor.authorAr Z.
dc.date.accessioned2024-07-22T08:12:12Z
dc.date.available2024-07-22T08:12:12Z
dc.date.issued2016
dc.description.abstractMigration, invasion, metastasis and angiogenesis associated with cancer depend on the surrounding microenvironment. Angiogenesis, the growth of new capillaries, is a regulator of cancer growth and a useful target for cancer therapy. We examined matrix protein interactions in a gastric cancer cell culture that was treated with different doses of caffeic acid (3,4-dihydroxycinnamic acid) phenethyl ester (CAPE). We also investigated the relations among the levels of vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), endostatin (ES) and trombospondin-1 (TSP-1). Cytotoxity of CAPE was measured using the 3-(4,5-dmethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. We examined the behavior of cells on laminin and collagen I coated surfaces in response to the angiogenic effect of these matrix molecules. We examined the protein alterations of these matrix molecules immunohistochemically and measured the levels of VEGF, MMP-9, ES and TSP-1 using the ELISA test. We showed that application of CAPE to the gastric cancer cell line on tissue culture plastic, laminin and collagen I significantly decreased the VEGF and MMP-9 protein levels. We found that TSP-1 levels were increased significantly in the gastric cancer cells after application of CAPE. The protein levels of gastric cancer cells also were increased significantly when tissue was cultured on laminin and collagen I. Application of CAPE to cells on laminin or collagen I coated surfaces significantly increased all of the proteins except ES. ES levels were increased on the collagen I covered surfaces, but the laminin surface decreased the levels of ES significantly. We demonstrated the beneficial effect of CAPE on a gastric cancer cell line including inhibition of proliferation and induction of some proteins that might be related to decreased angiogenesis. © 2015 The Biological Stain Commission.
dc.identifier.DOI-ID10.3109/10520295.2015.1072769
dc.identifier.issn10520295
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15965
dc.language.isoEnglish
dc.publisherTaylor and Francis Ltd
dc.subjectAngiogenesis Inducing Agents
dc.subjectAngiogenesis Inhibitors
dc.subjectCaffeic Acids
dc.subjectCell Line, Tumor
dc.subjectCell Proliferation
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectPhenylethyl Alcohol
dc.subjectStomach Neoplasms
dc.subjectangiogenesis inhibitor
dc.subjectangiogenic factor
dc.subjectcaffeic acid derivative
dc.subjectcaffeic acid phenethyl ester
dc.subjectphenethyl alcohol
dc.subjectanalogs and derivatives
dc.subjectcell proliferation
dc.subjectchemistry
dc.subjectdrug effects
dc.subjectenzyme linked immunosorbent assay
dc.subjecthuman
dc.subjectimmunohistochemistry
dc.subjectpathology
dc.subjectstomach tumor
dc.subjecttumor cell line
dc.titleEffects of caffeic acid phenethyl ester on matrix molecules and angiogenetic and anti-angiogenetic factors in gastric cancer cells cultured on different substrates
dc.typeArticle

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