Bone Healing in Rat Segmental Femur Defects with Graphene-PCL-Coated Borate-Based Bioactive Glass Scaffolds

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dc.contributor.authorBasal O.
dc.contributor.authorOzmen O.
dc.contributor.authorDeliormanlı A.M.
dc.date.accessioned2024-07-22T08:03:56Z
dc.date.available2024-07-22T08:03:56Z
dc.date.issued2022
dc.description.abstractBone is a continually regenerating tissue with the ability to heal after fractures, though healing significant damage requires intensive surgical treatment. In this study, borate-based 13-93B3 bioactive glass scaffolds were prepared though polymer foam replication and coated with a graphene-containing poly (ε-caprolactone) (PCL) layer to support bone repair and regeneration. The effects of graphene concentration (1, 3, 5, 10 wt%) on the healing of rat segmental femur defects were investigated in vivo using male Sprague–Dawley rats. Radiographic imaging, histopathological and immuno-histochemical (bone morphogenetic protein (BMP-2), smooth muscle actin (SMA), and alkaline phosphatase (ALP) examinations were performed 4 and 8 weeks after implantation. Results showed that after 8 weeks, both cartilage and bone formation were observed in all animal groups. Bone growth was significant starting from the 1 wt% graphene-coated bioactive glass-implanted group, and the highest amount of bone formation was seen in the group containing 10 wt% graphene (p < 0.001). Additionally, the presence of graphene nanoplatelets enhanced BMP-2, SMA and ALP levels compared to bare bioactive glass scaffolds. It was concluded that pristine graphene-coated bioactive glass scaffolds improve bone formation in rat femur defects. © 2022 by the authors.
dc.identifier.DOI-ID10.3390/polym14183898
dc.identifier.issn20734360
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12487
dc.language.isoEnglish
dc.publisherMDPI
dc.rightsAll Open Access; Gold Open Access; Green Open Access
dc.subjectBioactive glass
dc.subjectBone
dc.subjectGraphene
dc.subjectPhosphatases
dc.subjectRats
dc.subjectTissue regeneration
dc.subjectALkaline phosphatase
dc.subjectBone formation
dc.subjectBone healing
dc.subjectGlass scaffolds
dc.subjectIn-vivo
dc.subjectOsteogenesis
dc.subjectRat femur
dc.subjectRat femur defect
dc.subjectSmooth muscle actins
dc.subjectSurgical treatment
dc.subjectDefects
dc.titleBone Healing in Rat Segmental Femur Defects with Graphene-PCL-Coated Borate-Based Bioactive Glass Scaffolds
dc.typeArticle

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