Assessment of in-vitro antileishmanial activities of Cynara scolymus extracts against Leishmania tropica; [Leishmania tropica’ya karşı Cynara scolymus ekstrelerinin in-vitro antileishmanial etkinliğinin değerlendirilmesi]

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dc.contributor.authorYildirim A.
dc.contributor.authorAksoy T.
dc.contributor.authorCeylan Ş.S.
dc.contributor.authorKayalar H.
dc.contributor.authorTayfur E.
dc.contributor.authorBalcioğlu İ.C.
dc.date.accessioned2024-07-22T08:06:37Z
dc.date.available2024-07-22T08:06:37Z
dc.date.issued2021
dc.description.abstractIt was aimed to investigate in vitro antileishmanial activities of the receptacle, bractea, and stem leaves extracts of Cynara scolymus (artichoke) against Leishmania tropica. The Leishmania isolate, isolated from a cutaneous leishmaniasis patient from Manisa province, Turkey and stored in liquid nitrogen, was identified as L. tropica (MHOM/TR/2012/CBCL-LT) by genotyping. In vitro antileishmanial activities of C. scolymus plant extracts were examined by CelltTiter-glo and hemocytometry, and cytotoxic activities by MTT. IC50 values of receptacle water (WRC), aqueous ethanol (ARC) and ethanol (ERC), bractea leaf water (WBC), aqueous ethanol (ABC) and ethanol (EBC), and stem leaf water (WSC), aqueous ethanol (ASC) and ethanol (ESC) extracts were determined as 2.45 mg/mL, 1.52 mg/mL, 1.66 mg/mL, 3.45 mg/mL, 1.46 mg/mL and 0.58 mg/mL, 0.24 mg/mL, 0.21 mg/mL and 0.08 mg/mL, respectively. When these results are compared with the drug-free control group, it was determined that stem leaf aqueous ethanol (SI: 7.98), ethanol (SI: 4.96) and water (SI: 2.71) extracts with the highest selectivity index (SI) values showed antileishmanial activity (P<0.05). Extracts of C. scolymus did not show cytotoxic activity except for WBC, WRC and ARC. In conclusion, the data presented in the current study indicated that C. scolymus stem leaf extracts (ESC, ASC and WSC) present effective antileishmanial activity. Future studies could focus on the identification and purification of the antileishmanial compounds within these extracts for analysis of their in vivo antileishmanial activity. © 2021, Veteriner Fakultesi Dergisi. All rights reserved.
dc.identifier.DOI-ID10.9775/kvfd.2021.25656
dc.identifier.issn13006045
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13627
dc.language.isoEnglish
dc.publisherVeteriner Fakultesi Dergisi
dc.rightsAll Open Access; Gold Open Access
dc.subjectalcohol
dc.subjectamphotericin B
dc.subjectchlorogenic acid
dc.subjectCynara scolymus extract
dc.subjectechinocandin
dc.subjectflavonoid
dc.subjectfluconazole
dc.subjectinternal transcribed spacer 1
dc.subjectmiltefosine
dc.subjectolanzapine
dc.subjectparomomycin
dc.subjectplant extract
dc.subjectpraziquantel
dc.subjectantileishmanial activity
dc.subjectartichoke
dc.subjectArticle
dc.subjectCC50 (cytotoxic concentration)
dc.subjectcell viability
dc.subjectcutaneous leishmaniasis
dc.subjectcytotoxicity
dc.subjectechinococcosis
dc.subjectgenotype
dc.subjectIC50
dc.subjectin vitro study
dc.subjectin vivo study
dc.subjectLeishmania amazonensis
dc.subjectLeishmania donovani
dc.subjectLeishmania tropica
dc.subjectleishmaniasis
dc.subjectminimum inhibitory concentration
dc.subjectMTT assay
dc.subjectnonhuman
dc.subjectparasite load
dc.subjectplant leaf
dc.subjectplant stem
dc.subjectpromastigote
dc.subjectSchistosoma mansoni
dc.subjectschistosomiasis
dc.subjectScolymus
dc.subjectselectivity index
dc.subjecttoxicity testing
dc.subjecttropical disease
dc.subjectvisceral leishmaniasis
dc.titleAssessment of in-vitro antileishmanial activities of Cynara scolymus extracts against Leishmania tropica; [Leishmania tropica’ya karşı Cynara scolymus ekstrelerinin in-vitro antileishmanial etkinliğinin değerlendirilmesi]
dc.typeArticle

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