Attachment and growth of dental pulp stem cells on dentin in presence of extra calcium

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dc.contributor.authorÖzdal-Kurt F.
dc.contributor.authorŞen B.H.
dc.contributor.authorTuǧlu I.
dc.contributor.authorVatansever S.
dc.contributor.authorTürk B.T.
dc.contributor.authorDeliloǧlu-Gürhan I.
dc.date.accessioned2024-07-22T08:11:42Z
dc.date.available2024-07-22T08:11:42Z
dc.date.issued2016
dc.description.abstractObjective We aimed to differentiate dental pulp stem cells (DPSC) to odontoblast-like cells (ODPSC) and to investigate their attachment and growth on dentin in the presence of extra calcium by colorimetric assay and scanning electron microscopy (SEM). Methods After isolation of DPSC, they were differentiated to ODPSC. Standard dentin discs from human molar teeth were prepared. While the dentin discs in Group 1 did not receive any extra treatment, the discs in Group 2 were treated with acidic calcium phosphate precipitation (CPP) solution. In Group 3, the discs were suspended in phosphate buffered saline containing calcium. DPSC or ODPSC (3 × 104 cells/mL) were seeded on all discs and incubated for 7, 14 or 21 days. Attachment and growth of 7-day cell cultures on extra dentin samples were examined by SEM. MTT assay showed that number of cells on dentin surfaces was increased by time periods regardless of type of treatment and cells (p < 0.05). Results While DPSC and ODPSC showed similar proliferation rates at 7 and 14 days (p > 0.05), the number of ODPSC was higher than DPSC in 21-day samples (p = 0.039). MTT assay showed that number of cells on dentin surfaces was increased by time periods regardless of type of treatment and cells (p < 0.05). Calcium-treated dentin surfaces always had lower number of cells; being significant for only CPP-treated surfaces (p < 0.01). Both types of cells demonstrated good attachment and proliferation on dentin surfaces regardless of type of dentin treatment. Conclusions Because the nature of dentin surface itself showed good adhesive characteristics with ODPSC and DPSC, additional calcium treatment of dentin surfaces may not be necessary. © 2016 Published by Elsevier Ltd.
dc.identifier.DOI-ID10.1016/j.archoralbio.2016.04.008
dc.identifier.issn00039969
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15757
dc.language.isoEnglish
dc.publisherElsevier Ltd
dc.subjectCalcium
dc.subjectCalcium Phosphates
dc.subjectCell Adhesion
dc.subjectCell Differentiation
dc.subjectCell Proliferation
dc.subjectCells, Cultured
dc.subjectDental Pulp
dc.subjectDentin
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectMicroscopy, Electron, Scanning
dc.subjectMolar
dc.subjectOdontoblasts
dc.subjectSodium Chloride
dc.subjectStem Cells
dc.subjectSurface Properties
dc.subjectcalcium
dc.subjectcalcium phosphate
dc.subjectcalcium phosphate, monobasic
dc.subjectsodium chloride
dc.subjectcell adhesion
dc.subjectcell culture
dc.subjectcell differentiation
dc.subjectcell proliferation
dc.subjectchemistry
dc.subjectcytology
dc.subjectdentin
dc.subjectdrug effects
dc.subjecthuman
dc.subjectimmunohistochemistry
dc.subjectmetabolism
dc.subjectmolar tooth
dc.subjectodontoblast
dc.subjectphysiology
dc.subjectscanning electron microscopy
dc.subjectstem cell
dc.subjectsurface property
dc.subjecttooth pulp
dc.subjectultrastructure
dc.titleAttachment and growth of dental pulp stem cells on dentin in presence of extra calcium
dc.typeArticle

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