Detection of anti-leishmanial effect of the Lucilia sericata larval secretions in vitro and in vivo on Leishmania tropica: First work

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dc.contributor.authorPolat E.
dc.contributor.authorCakan H.
dc.contributor.authorAslan M.
dc.contributor.authorSirekbasan S.
dc.contributor.authorKutlubay Z.
dc.contributor.authorIpek T.
dc.contributor.authorOzbilgin A.
dc.date.accessioned2024-07-22T08:19:16Z
dc.date.available2024-07-22T08:19:16Z
dc.date.issued2012
dc.description.abstractIt is known that some of the enzymes and substances secreted by 2nd and 3rd stages of the Lucilia sericata larvae to have bacteriostatic and bactericidal effects. From this point of view, we investigated the anti-leishmanial effect of larval secretions of the L. sericata on the Leishmania tropica both in vitro and in vivo conditions.In vitro: It was observed that promastigotes of L. tropica had undergone lyzis within 1. min in the larval secretions of L. sericata. However, larval secretion was ineffective on the promastigotes within Novy-MacNeal-Nicolle (NNN) cultures and RPMI 1640 medium.In vivo: Seven groups of male Balb/C mice (6 study groups and 1 control group), each composed of eight weeks old 10 mice were formed. L. tropica promastigotes were injected subcutaneosly to the soles of the SG mice' feet. In study groups, cutaneous lesions were developed Limoncu et al., 1997 in 2 (20%) and 1 (10%) of the SG-1 and SG-2, respectively after 15. days. There were L. tropica in the smears prepared from the lesions and L. tropica was observed in the cultures. Cutaneous lesions were not developed in 8 (80%), 9 (90%) and 10 (100%) of the SG-I, SG-II and SG-III, respectively. There were no cutaneous lesions developed in the soles of the feet. There were no L. tropica in the smears prepared from the infected soles of the feet neither L. tropica was observed in the cultures.Larval secretions were given into the cutaneous lesions to the feet soles of the SG-IV, V and VI mice after 6. months. No healing was observed in the cutaneos lesions of 4 (40%), 5 (50%) and 1 (10%) of SG-IV, SG-V and SG-VI, after 6. months, respectively. There were L. tropica in the smears prepared from the lesions and L. tropica was observed in the cultures. On the other hand, the lesions of 6 (60%), 5 (50%) and 9 (90%) of SG-IV, SG-V and SG-VI were diminished in size and disappeared completely after 6. months. There were L. tropica observed in the smears prepared from the infected soles of the feet and no growth was observed in the cultures. In the smears prepared from the cutaneous lesions developed in the soles of the feet of the control group mice, L. tropica was visualized and observed in the cultures.A statistical significant difference was observed between study groups and control group (p<0.001).In our study we demonstrated for the first time that the secretions of the 2nd and 3rd stages sterile and pure larvae of L. sericata had effects on promastigotes of L. tropica in in vitro and very effective on amastigote forms in in vivo conditions. © 2012 Elsevier Inc.
dc.identifier.DOI-ID10.1016/j.exppara.2012.06.004
dc.identifier.issn10902449
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17598
dc.language.isoEnglish
dc.subjectAnimals
dc.subjectDiptera
dc.subjectLarva
dc.subjectLeishmania tropica
dc.subjectLeishmaniasis, Cutaneous
dc.subjectMale
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectLeishmania tropica
dc.subjectLucilia sericata
dc.subjectMus
dc.subjectSoleidae
dc.subjectnatural product
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal product
dc.subjectanimal tissue
dc.subjectantiprotozoal activity
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectdrug efficacy
dc.subjectdrug mechanism
dc.subjectfly
dc.subjectgenetic strain
dc.subjectin vitro study
dc.subjectin vivo study
dc.subjectlarval stage
dc.subjectLeishmania tropica
dc.subjectlucilia sericata
dc.subjectmaggot
dc.subjectmale
dc.subjectmouse
dc.subjectnonhuman
dc.subjectparasite cultivation
dc.subjectpriority journal
dc.subjectpromastigote
dc.subjectsecretion
dc.subjectskin leishmaniasis
dc.subjecttreatment outcome
dc.titleDetection of anti-leishmanial effect of the Lucilia sericata larval secretions in vitro and in vivo on Leishmania tropica: First work
dc.typeArticle

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