A novel association between TGFb1 and ADAMTS4 in coronary artery disease: A new potential mechanism in the progression of atherosclerosis and diabetes

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dc.contributor.authorUluçay S.
dc.contributor.authorÇam F.S.
dc.contributor.authorBatır M.B.
dc.contributor.authorSütçü R.
dc.contributor.authorBayturan Ö
dc.contributor.authorDemircan K.
dc.date.accessioned2024-07-22T08:12:58Z
dc.date.available2024-07-22T08:12:58Z
dc.date.issued2015
dc.description.abstractOBJECTIVE: Coronary artery disease is characterized by atherosclerosis in the vessel wall. Recently, it has been thought that increasing LDL-binding capacity of subendothelial proteoglycan fragments that are formed by protease activity can be responsible for the initiation of atherosclerosis. ADAMTS4 is a member of the versican-degrading proteinases. In vitro studies demonstrated that TGFb inhibits the expression of ADAMTS4 in macrophages. In this study, we aimed to investigate the role and association between TGFb1 and ADAMTS4 in coronary artery disease.; METHODS: A total of 84 cases with atheroma plaque and 72 controls without plaque were analyzed. The severity of disease was determined by Gensini score. TGFb1 gene polymorphisms were genotyped by the PCR-RFLP method. TGFb1 and ADAMTS4 serum levels were measured by ELISA method. Statistical analyses of genotypes and their relationship with serum levels were performed by chi-square, student t test and ANOVA.; RESULTS: ADAMTS4 levels were higher in cases compared with controls (p<0.05). In the patient group, ADAMTS4 levels were higher than in controls and correlated with TGFb1 serum levels (r=0.29; p<0.05) and severity of disease (r=0.20; p<0.05). The TGFb1 gene CCA haplotype was associated with 3.3-fold increase in coronary artery disease (OR=3.26 95% CI 1.22-8.68; p<0.05). Unexpectedly, ADAMTS4 serum levels were also higher in diabetic cases (p=0.05).; CONCLUSION: This study has demonstrated that ADAMTS4 may be responsible for the pathogenesis of atherosclerosis. This is the first report about the association between ADAMTS4 and TGFb1 serum levels in the progression of atherosclerosis in CAD. Furthermore, it is seen that TGFb1 haplotype can cause a genetic susceptibility to CAD in the Turkish population. To our knowledge, this is also the first report suggesting higher serum ADAMTS4 levels in diabetic patients.
dc.identifier.DOI-ID10.5152/akd.2014.5762
dc.identifier.issn21492271
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/16244
dc.language.isoEnglish
dc.rightsAll Open Access; Gold Open Access; Green Open Access
dc.subjectADAM Proteins
dc.subjectBiomarkers
dc.subjectCase-Control Studies
dc.subjectCoronary Artery Disease
dc.subjectDiabetes Mellitus, Type 2
dc.subjectDisease Progression
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectFemale
dc.subjectGenotype
dc.subjectHumans
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectPolymerase Chain Reaction
dc.subjectPolymorphism, Genetic
dc.subjectPolymorphism, Restriction Fragment Length
dc.subjectProcollagen N-Endopeptidase
dc.subjectROC Curve
dc.subjectSeverity of Illness Index
dc.subjectTransforming Growth Factor beta1
dc.subjectADAM protein
dc.subjectaggrecanase-1
dc.subjectbiological marker
dc.subjectprocollagen n proteinase
dc.subjecttransforming growth factor beta1
dc.subjectblood
dc.subjectcase control study
dc.subjectcomplication
dc.subjectcoronary artery disease
dc.subjectdisease course
dc.subjectenzyme linked immunosorbent assay
dc.subjectfemale
dc.subjectgenetic polymorphism
dc.subjectgenetics
dc.subjectgenotype
dc.subjecthuman
dc.subjectmale
dc.subjectmiddle aged
dc.subjectnon insulin dependent diabetes mellitus
dc.subjectpathophysiology
dc.subjectpolymerase chain reaction
dc.subjectreceiver operating characteristic
dc.subjectrestriction fragment length polymorphism
dc.subjectseverity of illness index
dc.titleA novel association between TGFb1 and ADAMTS4 in coronary artery disease: A new potential mechanism in the progression of atherosclerosis and diabetes
dc.typeArticle

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