Quercetine attenuates the gentamicin-induced ototoxicity in a rat model

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dc.contributor.authorSagit M.
dc.contributor.authorKorkmaz F.
dc.contributor.authorGürgen S.G.
dc.contributor.authorGundogdu R.
dc.contributor.authorAkcadag A.
dc.contributor.authorOzcan I.
dc.date.accessioned2024-07-22T08:12:39Z
dc.date.available2024-07-22T08:12:39Z
dc.date.issued2015
dc.description.abstractObjectives: The aim of this study is to evaluate the protective role of quercetin in gentamicin-induced ototoxicity through an auditory brainstem response (ABR) test and a histopathological evaluation of the cochlea. Methods: In this study, 48 female adult Sprague-Dawley rats aged 20-22 weeks and weighing 200-250. g were used. An ABR test was carried out on all rats prior to drug administration, after which, the rats were divided into four groups of 12 animals each. Drug administration was gentamicin 120. mg/kg plus ethanol in group one gentamicin 120. mg/kg plus quercetin 15. mg/kg in group two; quercetin 15. mg/kg in group three; and ethanol in group four. The drugs were administered intraperitoneally once a day for two weeks, and the ABR test was repeated after drug administration. Subsequently, the rats were sacrificed and their cochleae were dissected and examined histopathologically. Results: There was no significant difference between the pre-treatment ABR measurement values of the groups. However, a significant increase was detected in the ABR values in the group of rats that were administered gentamicin plus ethanol, while no statistically significant increase was found in the ABR values in the groups administered with gentamicin plus quercetin; quercetin alone and ethanol alone. The number of TUNEL positive cells in the inner and outer hair cells in the Corti organ was found to be fewer, and Caspase 3 and 9 expressions were found to be weaker in the group receiving gentamicin plus quercetin than in the group receiving gentamicin plus ethanol. Conclusions: Auditory function was detected to be significantly protected and apoptotic cells were found to be decreased when quercetin was administered together with gentamicin. From these results it was concluded that quercetin, a powerful antioxidant, attenuates ABR thresholds and histopathological lesions in the cochlea in gentamicin-induced ototoxicity in rats. © 2015 Elsevier Ireland Ltd.
dc.identifier.DOI-ID10.1016/j.ijporl.2015.09.023
dc.identifier.issn01655876
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/16139
dc.language.isoEnglish
dc.publisherElsevier Ireland Ltd
dc.subjectAnimals
dc.subjectAnti-Bacterial Agents
dc.subjectAntioxidants
dc.subjectApoptosis
dc.subjectCaspase 3
dc.subjectCaspase 9
dc.subjectEar Diseases
dc.subjectEvoked Potentials, Auditory, Brain Stem
dc.subjectFemale
dc.subjectGentamicins
dc.subjectHair Cells, Auditory, Inner
dc.subjectHair Cells, Auditory, Outer
dc.subjectQuercetin
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectcaspase 3
dc.subjectcaspase 9
dc.subjectgentamicin
dc.subjectquercetin
dc.subjectantiinfective agent
dc.subjectantioxidant
dc.subjectcaspase 3
dc.subjectcaspase 9
dc.subjectgentamicin
dc.subjectquercetin
dc.subjectadult
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectantioxidant activity
dc.subjectapoptosis
dc.subjectArticle
dc.subjectauditory system function
dc.subjectcell count
dc.subjectcell protection
dc.subjectcontrolled study
dc.subjectevoked brain stem auditory response
dc.subjectfemale
dc.subjecthistopathology
dc.subjectimmunohistochemistry
dc.subjectinner hair cell
dc.subjectnonhuman
dc.subjectototoxicity
dc.subjectouter hair cell
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectrat
dc.subjectTUNEL assay
dc.subjectanimal
dc.subjectchemically induced
dc.subjectchemistry
dc.subjectdrug effects
dc.subjectEar Diseases
dc.subjectpathology
dc.subjectSprague Dawley rat
dc.titleQuercetine attenuates the gentamicin-induced ototoxicity in a rat model
dc.typeArticle

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