[Cryopreservation of plasmodia with malaria models and establishment of a cryobank].; [Sitma modeli etkenleri ile kriyoprezervasyon çalişmalari ve kriyobanka oluşturulmasi.]

Özbilgin A.; Östan I.; Tabak T.; Aşar K.

[Cryopreservation of plasmodia with malaria models and establishment of a cryobank].; [Sitma modeli etkenleri ile kriyoprezervasyon çalişmalari ve kriyobanka oluşturulmasi.]

Date

2010

Authors

Abstract

Cryopreservation is simply a method of keeping living cells frozen with the chance of regaining cellular viability, functions and antigenic structures whenever required, after heating. In the present study, dimethyl sulphoxide (DMSO) was mixed with the red blood cells having 20% of parasitemia obtained from the mice infected with Plasmodium yoelii and Plasmodium berghei at a final concentration of 15%. For cryopreservation: both test tubes containing each Plasmodium species were kept 10 minutes at room temperature, 30 minutes at +4°C, 90 minutes at -20°C and finally at -80°C. Some were left at this temperature, while some were transferred into the liquid nitrogen tank at -196°C after being left at -80°C for three hours. Our observations and assessments demonstrated that both P. yoelii and P. berghei might keep their viability and virulence at -80°C and -196°C between the first and the sixth months of cryopreservation. It can be concluded that the cryopreservation of P. yoelii and P. berghei at -80°C and -196°C are successful, indicating the advantage of the establishment of parasite cryobanks in research laboratories.