Mucosal trauma induced apoptosis in guinea pig middle ear: Comparision of hemostatic agents

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dc.contributor.authorEren E.
dc.contributor.authorBaşoğlu M.S.
dc.contributor.authorKulduk E.
dc.contributor.authorŞimsek F.
dc.contributor.authorInan S.
dc.date.accessioned2024-07-22T08:14:31Z
dc.date.available2024-07-22T08:14:31Z
dc.date.issued2014
dc.description.abstractObjective: The aim of this study is to compare the effects of the absorbable gelatin sponge (AGS), microporous polysaccharide hemospheres (MPH), and Ankaferd on wound healing after middle ear trauma and to evaluate their ototoxicity in an experimental guinea pig model. Methods: Middle ear mucosal trauma was created in 21 healthy adult guinea pigs. MPH, Ankaferd, and AGS were applied into the right tympanic bulla of the guinea pigs (7 ears for each treatment modality). The left ears of the seven animals were used as the sham group. At the fourth postoperative week (28-30 days), the guinea pigs were decapitated. Apoptosis was investigated, and the expression of Bcl-xl, Apaf, p53, cytochrome 3, and caspase 3 were evaluated. Results: The Ankaferd and AGS groups demonstrated significantly lower epithelial thickness, inflammation, and capillary dilatation than did the control group ( p<. 0.001, <0.001, /0.001, <0.001/, 0.005, and 0.005, respectively). A statistically significant decrease in Bcl-xl staining was observed in the middle ears of animals treated with MPH ( p= 0.003). There was significantly higher caspase 3 expression in the Ankaferd and AGS groups than in the control group ( p<. 0.001 and p= 0.002, respectively). Conclusion: Light microscopy indicates that Ankaferd and AGS create less inflammation and increased caspase expression, which seems to induce inflammatory cell apoptosis. Ankaferd seems to be a promising hemostatic agent in otology. © 2014 Elsevier Ireland Ltd.
dc.identifier.DOI-ID10.1016/j.ijporl.2014.10.017
dc.identifier.issn01655876
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/16597
dc.language.isoEnglish
dc.publisherElsevier Ireland Ltd
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectbcl-X Protein
dc.subjectBiocompatible Materials
dc.subjectCaspase 3
dc.subjectEar, Middle
dc.subjectEpithelium
dc.subjectGelatin Sponge, Absorbable
dc.subjectGuinea Pigs
dc.subjectHemostatics
dc.subjectInflammation
dc.subjectMicroscopy
dc.subjectModels, Animal
dc.subjectMucous Membrane
dc.subjectPlant Extracts
dc.subjectPolysaccharides
dc.subjectWound Healing
dc.subjectankaferd blood stopper
dc.subjectapoptotic protease activating factor 1
dc.subjectcaspase 3
dc.subjectcytochrome
dc.subjectcytochrome 3
dc.subjectgelatin sponge
dc.subjecthemostatic agent
dc.subjectmicroporous polysaccharide hemosphere
dc.subjectplant extract
dc.subjectprotein bcl xl
dc.subjectprotein p53
dc.subjectunclassified drug
dc.subjectankaferd blood stopper
dc.subjectbiomaterial
dc.subjectcaspase 3
dc.subjectgelatin sponge
dc.subjecthemostatic agent
dc.subjectplant extract
dc.subjectpolysaccharide
dc.subjectprotein bcl x
dc.subjectadult
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectapoptosis
dc.subjectArticle
dc.subjectcapillary
dc.subjectcontrolled study
dc.subjectdrug effect
dc.subjectepithelium
dc.subjectexperimental model
dc.subjectguinea pig
dc.subjectguinea pig model
dc.subjectinflammation
dc.subjectinflammatory cell
dc.subjectmicroscopy
dc.subjectmiddle ear
dc.subjectmiddle ear disease
dc.subjectmiddle ear mucosa
dc.subjectmiddle ear trauma
dc.subjectmiddle ear trauma
dc.subjectnonhuman
dc.subjectototoxicity
dc.subjectprotein expression
dc.subjectstaining
dc.subjectthickness
dc.subjectvasodilatation
dc.subjectwound healing
dc.subjectanimal
dc.subjectapoptosis
dc.subjectcomparative study
dc.subjectdrug effects
dc.subjectinjuries
dc.subjectmetabolism
dc.subjectmucosa
dc.subjectpathology
dc.titleMucosal trauma induced apoptosis in guinea pig middle ear: Comparision of hemostatic agents
dc.typeArticle

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