IL-5, IL-8 and MMP -9 levels in exhaled breath condensate of atopic and nonatopic asthmatic children

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dc.contributor.authorTurkeli A.
dc.contributor.authorYilmaz O.
dc.contributor.authorTaneli F.
dc.contributor.authorHorasan G.D.
dc.contributor.authorKanik E.T.
dc.contributor.authorKizilkaya M.
dc.contributor.authorGozukara C.
dc.contributor.authorYuksel H.
dc.date.accessioned2024-07-22T08:13:18Z
dc.date.available2024-07-22T08:13:18Z
dc.date.issued2015
dc.description.abstractRationale Asthma is a heterogeneous disease, and a great majority of pediatric patients with asthma demonstrate atopic characteristics and develop a Th2 type cytokine response. Nonatopic asthma, on the other hand, is seen more rarely. Methods In this study, levels of IL-5, IL-8 and MMP-9 were measured in exhaled breath condensate (EBC) of the subjects to demonstrate the extent of tissue damage as well as eosinophilic and neutrophilic inflammation in children with atopic and nonatopic asthma. A total of 37 children with atopic asthma and 37 children with nonatopic asthma were enrolled in the study. Patients who exhibited protease positive aeroallergen (House dust mite, mould mix, olea, grass mix) sensitivity in allergen skin prick test were included in the atopic asthma group. To evaluate the EBC, the fluid content of the breath was collected by having the patients exhale into an EBC device, after which the IL-5, IL-8 and MMP-9 levels were assayed using the ELISA method. Results The atopic asthmatics exhibited significantly higher IL-5 levels in their EBC samples than the nonatopic asthmatics (0.271 [0.198-0.489] pg/ml and 0.198 [0.125-0.344] pg/ml, respectively, p = 0.04), while no significant differences were observed in the levels of IL-8 and MMP-9 in the EBC samples of the atopic and nonatopic asthmatics. Conclusions IL-5 levels, as a marker of eosinophilic inflammation, were demonstrated to be higher in the children with atopic asthma when compared to those with nonatopic asthma in EBC. The fact that no significant difference was apparent in the IL-8 levels between the groups suggests that it is the severity of the disease rather than the atopic state that plays an important role in IL-8 levels. Since no difference was recorded between the groups in terms of MMP-9 levels, lung damage in asthma sufferers seems to develop independent of atopia. © 2015 Elsevier Ltd.
dc.identifier.DOI-ID10.1016/j.rmed.2015.04.004
dc.identifier.issn09546111
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/16317
dc.language.isoEnglish
dc.publisherW.B. Saunders Ltd
dc.rightsAll Open Access; Hybrid Gold Open Access
dc.subjectAsthma
dc.subjectBiomarkers
dc.subjectBreath Tests
dc.subjectCase-Control Studies
dc.subjectChild
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectFemale
dc.subjectHumans
dc.subjectInterleukin-5
dc.subjectInterleukin-8
dc.subjectMale
dc.subjectMatrix Metalloproteinase 9
dc.subjectTh2 Cells
dc.subjectTurkey
dc.subjectgelatinase B
dc.subjecthouse dust allergen
dc.subjectimmunoglobulin E
dc.subjectinterleukin 5
dc.subjectinterleukin 8
dc.subjectpollen antigen
dc.subjectproteinase
dc.subjectbiological marker
dc.subjectgelatinase B
dc.subjectIL5 protein, human
dc.subjectIL8 protein, human
dc.subjectinterleukin 5
dc.subjectinterleukin 8
dc.subjectMMP9 protein, human
dc.subjectallergic asthma
dc.subjectallergy test
dc.subjectArticle
dc.subjectasthma
dc.subjectbreath condensate
dc.subjectchild
dc.subjectcontrolled study
dc.subjectdisease severity
dc.subjectenzyme linked immunosorbent assay
dc.subjecteosinophil count
dc.subjecteosinophilia
dc.subjectexpired air
dc.subjectfemale
dc.subjectgrass
dc.subjecthuman
dc.subjectimmunoglobulin blood level
dc.subjectlung function test
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectmild intermittent asthma
dc.subjectmild persistent asthma
dc.subjectmoderate persistent asthma
dc.subjectmould
dc.subjectneutrophilia
dc.subjectolive tree
dc.subjectprick test
dc.subjectpriority journal
dc.subjectrespiratory tract inflammation
dc.subjectschool child
dc.subjectsevere persistent asthma
dc.subjectskin test
dc.subjecttissue injury
dc.subjectasthma
dc.subjectbreath analysis
dc.subjectcase control study
dc.subjectimmunology
dc.subjectmetabolism
dc.subjectpathology
dc.subjectTh2 cell
dc.subjectTurkey
dc.titleIL-5, IL-8 and MMP -9 levels in exhaled breath condensate of atopic and nonatopic asthmatic children
dc.typeArticle

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