Essential Oils from Vitex agnus castus L. Leaves Induces Caspase-Dependent Apoptosis of Human Multidrug-Resistant Lung Carcinoma Cells through Intrinsic and Extrinsic Pathways

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dc.contributor.authorIlhan S.
dc.date.accessioned2024-07-22T08:08:01Z
dc.date.available2024-07-22T08:08:01Z
dc.date.issued2020
dc.description.abstractEssential oil (EO) fractions of plants are complex mixtures of volatile compounds with broad-spectrum biological properties. In the current study, the EO content of Vitex agnus castus L. (VAC) leaves growing in the Aegean region of Turkey was extracted and identified. Then, VAC EOs were investigated for their potential antioxidant, cytotoxic and apoptotic effects in human H69AR multi-drug resistant cancer cells. EOs were isolated by hydrodistillation and chemical composition was determined by GC-MS. Cell viability was assessed via MTT and trypan blue assays. Antioxidant activity was evaluated by measuring the total antioxidant activity and free radical scavenging activity. Apoptosis was evaluated via DNA fragmentation and caspase 3/7 activity assays. Changes in the levels of apoptotic genes were determined by RT-qPCR. The results indicated strong antioxidant activity and cytotoxic effect on H69AR cancer cells but not on HEK-293 human normal cells indicating the tumor-specific effect. VAC EOs induced caspase 3/7 activation and apoptosis through triggering both extrinsic- and intrinsic-pathways by modulating Bcl-2, Bcl-XL, Bax, Bad, FADD, Caspase-8, Caspase-9, TRAIL R1/DR4 and TRAIL R2/DR5. This study revealed that VAC EOs may be a promising candidate in the development of novel therapeutic agents for multi-drug resistant lung cancer treatment. © 2020 Taylor & Francis Group, LLC.
dc.identifier.DOI-ID10.1080/01635581.2020.1823439
dc.identifier.issn01635581
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14216
dc.language.isoEnglish
dc.publisherRoutledge
dc.subjectApoptosis
dc.subjectCarcinoma
dc.subjectDrug Resistance, Multiple
dc.subjectDrug Resistance, Neoplasm
dc.subjectHEK293 Cells
dc.subjectHumans
dc.subjectLung
dc.subjectLung Neoplasms
dc.subjectOils, Volatile
dc.subjectPlant Leaves
dc.subjectVitex
dc.subjectcaspase 3
dc.subjectcaspase 7
dc.subjectcaspase 8
dc.subjectcaspase 9
dc.subjectdeath receptor 4
dc.subjectdeath receptor 5
dc.subjectessential oil
dc.subjectFas associated death domain protein
dc.subjectprotein BAD
dc.subjectprotein Bax
dc.subjectprotein bcl 2
dc.subjectprotein bcl xl
dc.subjectscavenger
dc.subjectVitex agnus castus extract
dc.subjectessential oil
dc.subjectantioxidant activity
dc.subjectapoptosis
dc.subjectArticle
dc.subjectcell viability
dc.subjectchemical composition
dc.subjectcontrolled study
dc.subjectcytotoxicity
dc.subjectDNA fragmentation
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthydrodistillation
dc.subjectlung cancer
dc.subjectlung carcinoma cell line
dc.subjectmass fragmentography
dc.subjectmRNA expression level
dc.subjectplant leaf
dc.subjectreal time polymerase chain reaction
dc.subjecttime-dependent inhibition
dc.subjecttrypan blue assay
dc.subjectapoptosis
dc.subjectcarcinoma
dc.subjectdrug resistance
dc.subjectHEK293 cell line
dc.subjectlung
dc.subjectlung tumor
dc.subjectmultidrug resistance
dc.subjectplant leaf
dc.subjectVitex
dc.titleEssential Oils from Vitex agnus castus L. Leaves Induces Caspase-Dependent Apoptosis of Human Multidrug-Resistant Lung Carcinoma Cells through Intrinsic and Extrinsic Pathways
dc.typeArticle

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