Immobilization of catalase on chitosan and amino acid-modified chitosan beads

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dc.contributor.authorBaşak E.
dc.contributor.authorAydemir T.
dc.date.accessioned2024-07-22T08:18:05Z
dc.date.available2024-07-22T08:18:05Z
dc.date.issued2013
dc.description.abstractBovine liver catalase was covalently immobilized onto amino acid-modified chitosan beads. The beads were characterized with SEM, FTIR, TGA and the effects of immobilization on optimum pH and temperature, thermostability, reusability were evaluated. Immobilized catalase showed the maximal enzyme activity at pH 7.0 at 30°C. The kinetic parameters, Km and Vmax, for immobilized catalase on alanine-chitosan beads and lysine-chitosan beads were estimated to be 25.67 mM, 27 mM and 201.39 μmol H2O 2/min, 197.50 μmol H2O2/min, respectively. The activity of the immobilized catalase on Ala-CB and Lys-CB retained 40% of its high initial activity after 100 times of reuse. © 2013 Informa Healthcare USA, Inc.
dc.identifier.DOI-ID10.3109/10731199.2012.742095
dc.identifier.issn2169141X
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17173
dc.language.isoEnglish
dc.subjectAlanine
dc.subjectAnimals
dc.subjectCatalase
dc.subjectCattle
dc.subjectChitosan
dc.subjectEnzyme Stability
dc.subjectEnzymes, Immobilized
dc.subjectFood Industry
dc.subjectGlutaral
dc.subjectKinetics
dc.subjectLysine
dc.subjectMicrospheres
dc.subjectTemperature
dc.subjectAcids
dc.subjectChitosan
dc.subjectRadioactive waste vitrification
dc.subjectReusability
dc.subjectalanine
dc.subjectamino acid
dc.subjectcatalase
dc.subjectchitosan
dc.subjectlysine
dc.subjectnanobead
dc.subjectAmino
dc.subjectBovine liver catalase
dc.subjectCatalase
dc.subjectChitosan beads
dc.subjectFTIR
dc.subjectInitial activity
dc.subjectModification
dc.subjectOptimum pH
dc.subjectarticle
dc.subjectchemical modification
dc.subjectenzyme activity
dc.subjectenzyme immobilization
dc.subjectenzyme kinetics
dc.subjectinfrared spectroscopy
dc.subjectpH
dc.subjectscanning electron microscopy
dc.subjecttemperature
dc.subjectthermostability
dc.subjectAmino acids
dc.titleImmobilization of catalase on chitosan and amino acid-modified chitosan beads
dc.typeArticle

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