Impact of Pregnancy and Glucocorticoid Treatment on NF-κB and MUC5AC in Mucosa of Rat Larynx

Simple item page
dc.contributor.authorUlkumen B.
dc.contributor.authorArtunc Ulkumen B.
dc.contributor.authorBatir M.B.
dc.contributor.authorPala H.G.
dc.contributor.authorVatansever S.
dc.contributor.authorCam S.
dc.date.accessioned2024-07-22T08:06:12Z
dc.date.available2024-07-22T08:06:12Z
dc.date.issued2021
dc.description.abstractObjective: The aim of this study is to reveal physiological expression and distribution of nuclear factor-kappa B (NF-κB) and MUC 5 subtype AC (MUC5AC) in rat laryngeal mucosa and to find out the effect of pregnancy and glucocorticoid treatment on these biomolecules. Methods: This animal experiment was done in Experimental Animals Research and Application Center of Manisa Celal Bayar University in accordance with the accepted policy on the use of animals. A total of 30 young, adult Wister albino female rats were randomized into a control group (group A), a pregnant group (group B), and a steroid administered group (group C). Sacrification was done by injection of sodium-pentobarbitone (400 mg/kg) solution via intraperitoneal route in all groups. Serum estradiole (E2) and progesterone (PG) were determined by enzyme-linked immunosorbent assay. The relative expression and distribution of NF-κB and MUC5AC in laryngeal mucosa was studied both by immunohistochemistry (IHC) and polymerase chain reaction testing. Expression and immunohistochemical localization of NF-κB and MUC5AC was evaluated by light microscopy (Olympus BX41). In statistical analyses; relative expression of NF-κB and MUC5AC were compared on group basis. The effect of E2 and PG levels on these biomolecules was also evaluated. Results: NF-κB was found to be significantly low both in group B (P < 0.05) and C (P < 0.001) when compared with group A, while MUC5AC was found to be significantly high both in group B (P < 0.05) and group C (P < 0.05) when compared with group A. Concerning IHC; NF-κB was found to be expressed in epithelium and lamina propria. MUC5AC was found to be expressed particularly in the epithelial layer in all groups. Statistically significant negative correlation between PG and NF-κB expression (P = 0.048), but no correlation between PG and MUC5AC expression (P = 0.487) were revealed. On the other hand, no correlation was found between E2 and the expression of relevant biomolecules (NF-κB [P = 0.270], MUC5AC [P = 0.829]). We also did found a significant negative correlation between the expression of NF-κB and MUC5AC (P = 0.031). Conclusions: In this study, the physiological expression of NF-κB and MUC5AC in rat laryngeal mucosa was shown for the first time both by polymerase chain reaction and IHC. The impact of pregnancy and glucocorticoid treatment on the expression and distribution of these biomolecules was also revealed. The expression of NF-κB was found to be decreased while the expression of MUC5AC was found to be increased both by pregnancy and glucocorticoid treatment. The inhibitory effect of serum PG on NF-κB expression in rat laryngeal mucosa was also shown for the first time. The expression of MUC5AC was found to be increased both in pregnant and glucocorticoid administered group. Negative correlation between NF-κB and MUC5AC expression was also revealed in rat larynx for the first time. These findings may partially unclose the histochemical background of voice changes caused by pregnancy and as well as by glucocorticoid treatment. © 2019 The Voice Foundation
dc.identifier.DOI-ID10.1016/j.jvoice.2019.11.008
dc.identifier.issn08921997
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13422
dc.language.isoEnglish
dc.publisherMosby Inc.
dc.subjectAnimals
dc.subjectFemale
dc.subjectGlucocorticoids
dc.subjectLarynx
dc.subjectMucin 5AC
dc.subjectMucous Membrane
dc.subjectNF-kappa B
dc.subjectPregnancy
dc.subjectRats
dc.subjectbeta 2 microglobulin
dc.subjectestradiol
dc.subjectglucocorticoid
dc.subjecthypoxanthine phosphoribosyltransferase
dc.subjectimmunoglobulin enhancer binding protein
dc.subjectmometasone furoate
dc.subjectmucin 5AC
dc.subjectpentobarbital
dc.subjectprogesterone
dc.subjectRNA
dc.subjectsex hormone
dc.subjectglucocorticoid
dc.subjectimmunoglobulin enhancer binding protein
dc.subjectMuc5ac protein, rat
dc.subjectmucin 5AC
dc.subjectadult
dc.subjectalbino rat
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectcontrolled study
dc.subjectcorticosteroid therapy
dc.subjectdysphonia
dc.subjectfemale
dc.subjecthistopathology
dc.subjectimmunohistochemistry
dc.subjectlamina propria
dc.subjectlarynx mucosa
dc.subjectmicroscopy
dc.subjectnonhuman
dc.subjectpolymerase chain reaction
dc.subjectpregnancy
dc.subjectprogesterone blood level
dc.subjectprotein expression
dc.subjectrat
dc.subjectvocal cord
dc.subjectvoice change
dc.subjectanimal
dc.subjectgenetics
dc.subjectlarynx
dc.subjectmetabolism
dc.subjectmucosa
dc.subjectpregnancy
dc.titleImpact of Pregnancy and Glucocorticoid Treatment on NF-κB and MUC5AC in Mucosa of Rat Larynx
dc.typeArticle

Files

Collections

Scopus Koleksiyonu