Immunohistochemical detection of transforming growth factor-α, epidermal growth factor, and vascular endothelial growth factor expression in hyperstimulated rat ovary

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dc.contributor.authorOzcakir H.T.
dc.contributor.authorGiray S.G.
dc.contributor.authorOzbilgin M.K.
dc.contributor.authorUyar Y.
dc.contributor.authorLacin S.
dc.contributor.authorCaglar H.
dc.date.accessioned2024-07-22T08:23:52Z
dc.date.available2024-07-22T08:23:52Z
dc.date.issued2005
dc.description.abstractObjective. The aim of the present study is to figure out the immunohistochemical expression of transforming growth factor-α (TGF-α), epidermal growth factor (EGF), and vascular endothelial growth factor (VEGF) in hyperstimulated rat ovaries. Methods. Twenty Wistar-Albino adult female rats (250-300 g) were taken into the study. The animals were randomly divided into two groups, each containing 10 rats: (i) stimulation group and (ii) control group. In the stimulation group, a stimulation regimen was administered to induce follicular maturity and ovarian hyperstimulation syndrome (OHSS) at the end using a 30-IU follicle-stimulating hormone that was administered subcutaneously for 4 consecutive days, followed by a 30-IU human chorionic gonadotropin on day 5 to induce ovulation. The rats, in the control group, received 0.2ml of 0.9% NaCl for 5 consecutive days to mimic the conditions of the study animals. At the end of the treatment period, all rats underwent ovariectomy and the sections of ovaries were stained for the TGF-α, EGF, and VEGF. Results. The expression of TGF-α, EGF, and VEGF in the endothelium, the stroma, the granulosa cells, and the corpus luteum was found to be significantly higher in the stimulated group, compared to that in the control group (p < 0.05). Conclusion. TGF-α, EGF, and VEGF are found to have increased in the hyperstimulated ovaries and this finding seems to be involved in the OHSS pathogenesis. © Acta Obstet Gynecol Scand 2005.
dc.identifier.DOI-ID10.1111/j.0001-6349.2005.00586.x
dc.identifier.issn00016349
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19729
dc.language.isoEnglish
dc.rightsAll Open Access; Bronze Open Access
dc.subjectAnimals
dc.subjectChorionic Gonadotropin
dc.subjectEpidermal Growth Factor
dc.subjectFemale
dc.subjectFollicle Stimulating Hormone
dc.subjectHormones
dc.subjectImmunohistochemistry
dc.subjectModels, Animal
dc.subjectOrgan Size
dc.subjectOvarian Hyperstimulation Syndrome
dc.subjectOvary
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectTransforming Growth Factor alpha
dc.subjectVascular Endothelial Growth Factor A
dc.subjectepidermal growth factor
dc.subjectfollitropin
dc.subjecthuman placenta lactogen
dc.subjectsodium chloride
dc.subjecttransforming growth factor alpha
dc.subjectvasculotropin
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectendothelium cell
dc.subjectfemale
dc.subjectgranulosa cell
dc.subjectimmunohistochemistry
dc.subjectluteal cell
dc.subjectnonhuman
dc.subjectovariectomy
dc.subjectovary
dc.subjectovary disease
dc.subjectovary follicle
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectrat
dc.subjectstimulation
dc.subjectstroma cell
dc.titleImmunohistochemical detection of transforming growth factor-α, epidermal growth factor, and vascular endothelial growth factor expression in hyperstimulated rat ovary
dc.typeArticle

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