Surface Area of Graphene Governs Its Neurotoxicity

dc.contributor.authorTaşdemir Ş.
dc.contributor.authorMorçimen Z.G.
dc.contributor.authorDoǧan A.A.
dc.contributor.authorGörgün C.
dc.contributor.authorŞendemir A.
dc.date.accessioned2024-07-22T08:02:32Z
dc.date.available2024-07-22T08:02:32Z
dc.date.issued2023
dc.description.abstractDue to their unique physicochemical properties, graphene and its derivatives are widely exploited for biomedical applications. It has been shown that graphene may exert different degrees of toxicity in in vivo or in vitro models when administered via different routes and penetrated through physiological barriers, subsequently being distributed within tissues or located within cells. In this study, in vitro neurotoxicity of graphene with different surface areas (150 and 750 m2/g) was examined on dopaminergic neuron model cells. SH-SY5Y cells were treated with graphene possessing two different surface areas (150 and 750 m2/g) in different concentrations between 400 and 3.125 μg/mL, and the cytotoxic and genotoxic effects were investigated. Both sizes of graphene have shown increased cell viability in decreasing concentrations. Cell damage increased with higher surface area. Lactate dehydrogenase (LDH) results have concluded that the viability loss of the cells is not through membrane damage. Neither of the two graphene types showed damage through lipid peroxidation (MDA) oxidative stress pathway. Glutathione (GSH) values increased within the first 24 and 48 h for both types of graphene. This increase suggests that graphene has an antioxidant effect on the SH-SY5Y model neurons. Comet analysis shows that graphene does not show genotoxicity on either surface area. Although there are many studies on graphene and its derivatives on their use with different cells in the literature, there are conflicting results in these studies, and most of the literature is focused on graphene oxide. Among these studies, no study examining the effect of graphene surface areas on the cell was found. Our study contributes to the literature in terms of examining the cytotoxic and genotoxic behavior of graphene with different surface areas. © 2023 American Chemical Society. All rights reserved.
dc.identifier.DOI-ID10.1021/acsbiomaterials.3c00104
dc.identifier.issn23739878
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/11876
dc.language.isoEnglish
dc.publisherAmerican Chemical Society
dc.rightsAll Open Access; Green Open Access; Hybrid Gold Open Access
dc.subjectAntioxidants
dc.subjectCell Line, Tumor
dc.subjectGlutathione
dc.subjectGraphite
dc.subjectHumans
dc.subjectNeuroblastoma
dc.subjectOxidative Stress
dc.subjectCells
dc.subjectCytology
dc.subjectMedical applications
dc.subjectNeurons
dc.subjectPhysicochemical properties
dc.subjectPhysiological models
dc.subjectToxicity
dc.subjectantioxidant
dc.subjectcaspase 3
dc.subjectcaspase 7
dc.subjectglutathione
dc.subjectgraphene
dc.subjectgraphene oxide
dc.subjectlactate dehydrogenase
dc.subjectlipid
dc.subjectneuroprotective agent
dc.subjectreactive oxygen metabolite
dc.subjectantioxidant
dc.subjectglutathione
dc.subjectgraphite
dc.subjectBiomedical applications
dc.subjectCell-be
dc.subjectCell/B.E
dc.subjectCell/BE
dc.subjectGenotoxicities
dc.subjectIn-vivo
dc.subjectNeurotoxicity
dc.subjectPhysicochemical property
dc.subjectSH-SY5Y
dc.subjectSurface area
dc.subjectantioxidant activity
dc.subjectapoptosis
dc.subjectArticle
dc.subjectcell damage
dc.subjectcell viability
dc.subjectcomet assay
dc.subjectconcentration (parameter)
dc.subjectcontrolled study
dc.subjectcytotoxicity
dc.subjectDNA damage
dc.subjectDNA fragmentation
dc.subjectendosome
dc.subjectgenetic manipulation
dc.subjectgenotoxicity
dc.subjecthuman
dc.subjecthuman cell
dc.subjectin vitro study
dc.subjectlipid peroxidation
dc.subjectmembrane damage
dc.subjectnerve cell
dc.subjectneuroprotection
dc.subjectneurotoxicity
dc.subjectoxidative stress
dc.subjectphagosome
dc.subjectSH-SY5Y cell line
dc.subjectsurface area
dc.subjectsynaptic transmission
dc.subjectultraviolet radiation
dc.subjectmetabolism
dc.subjectneuroblastoma
dc.subjecttumor cell line
dc.subjectGraphene
dc.titleSurface Area of Graphene Governs Its Neurotoxicity
dc.typeArticle

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