Protective effect of ketamine against hemorrhagic cystitis in rats receiving ifosfamide

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dc.contributor.authorOzguven A.A.
dc.contributor.authorYilmaz O.
dc.contributor.authorTaneli F.
dc.contributor.authorUlman C.
dc.contributor.authorVatansever S.
dc.contributor.authorOnag A.
dc.date.accessioned2024-07-22T08:17:28Z
dc.date.available2024-07-22T08:17:28Z
dc.date.issued2014
dc.description.abstractObjective: To investigate the possible protective effect of a single dose of ketamine and the synergistic effect between ketamine and 2-mercaptoethane sulfonate (mesna) against ifosfamide-induced hemorrhagic cystitis. Materials and Methods: 35 adult female wistar rats were divided into five groups and pretreated with ketamine at 10 mg/kg and/or mesna 400 mg/kg 30 minutes before intraperitoneal injection of IFS (400 mg/kg) or with saline (control group). Hemorrhagic cystitis was evaluated 24 hours after IFS injection according to bladder wet weight (BWW), and microscopic changes, i.e. edema, hemorrhage, cellular infiltration, and urothelial desquamation. The markers of oxidative damage including nitric oxide (NO) and malondialdehyde (MDA) levels and the expressions of tumor necrosis factor alpha (TNF-α), interleukin 1-beta (IL-1β), inducible nitric oxide synthase (i-NOS) and endothelial nitric oxide synthase (e-NOS) were also assayed in the bladder tissues. Results: Pretreatment with ketamine alone or ketamine in combination with mesna reduced the IFS-induced increase of BWW (58,47% and 63,33%, respectively, P < 0.05). IFS- induced microscopic alterations were also prevented by ketamine with or without mesna (P < 0.05). In addition, also statistically insignificant, the bladder tissue expressions of IL-1β were lower in ketamine and/or mesna-receiving groups (P > 0,05). The parameters of oxidative stress, the NO and the MDA contents of the bladder tissues of the study groups were not different. Conclusion: The results of the present study suggest that a single dose of ketamine pretreatment attenuates experimental IFS-induced bladder damage. It is therefore necessary to investigate ketamine locally and systematically with various dosing schedulesin order to reduce the bladder damage secondary to oxazaphosphorine-alkylating agents and these results may widen the spectrum of ketamine.
dc.identifier.DOI-ID10.4103/0253-7613.129301
dc.identifier.issn02537613
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17064
dc.language.isoEnglish
dc.publisherMedknow Publications
dc.rightsAll Open Access; Green Open Access
dc.subjectAnimals
dc.subjectAntineoplastic Agents, Alkylating
dc.subjectBiological Markers
dc.subjectCystitis
dc.subjectDrug Therapy, Combination
dc.subjectFemale
dc.subjectHemorrhage
dc.subjectIfosfamide
dc.subjectImmunohistochemistry
dc.subjectKetamine
dc.subjectMesna
dc.subjectOrgan Size
dc.subjectOxidative Stress
dc.subjectRats, Wistar
dc.subjectUrinary Bladder
dc.subjectendothelial nitric oxide synthase
dc.subjectifosfamide
dc.subjectinducible nitric oxide synthase
dc.subjectinterleukin 1beta
dc.subjectketamine
dc.subjectmalonaldehyde
dc.subjectmesna
dc.subjectnitric oxide
dc.subjecttumor necrosis factor alpha
dc.subjectalkylating agent
dc.subjectbiological marker
dc.subjectifosfamide
dc.subjectketamine
dc.subjectmesna
dc.subjectadult
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectbladder injury
dc.subjectbladder wet weight
dc.subjectcontrolled study
dc.subjectdrug potentiation
dc.subjectedema
dc.subjectfemale
dc.subjecthemorrhagic cystitis
dc.subjecthistopathology
dc.subjectnonhuman
dc.subjectoxidative stress
dc.subjectpremedication
dc.subjectprotein expression
dc.subjectrat
dc.subjectrenal protection
dc.subjectrenal system parameters
dc.subjectsingle drug dose
dc.subjectvesical edema
dc.subjectanimal
dc.subjectbladder
dc.subjectchemically induced
dc.subjectcystitis
dc.subjectdrug combination
dc.subjectdrug effects
dc.subjectHemorrhage
dc.subjectimmunohistochemistry
dc.subjectmetabolism
dc.subjectorgan size
dc.subjectpathology
dc.subjecttoxicity
dc.subjectWistar rat
dc.titleProtective effect of ketamine against hemorrhagic cystitis in rats receiving ifosfamide
dc.typeArticle

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