Propolis from Turkey induces apoptosis through activating caspases in human breast carcinoma cell lines

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dc.contributor.authorSeda Vatansever H.
dc.contributor.authorSorkun K.
dc.contributor.authorIsmet Deliloĝlu Gurhan S.
dc.contributor.authorOzdal-Kurt F.
dc.contributor.authorTurkoz E.
dc.contributor.authorGencay O.
dc.contributor.authorSalih B.
dc.date.accessioned2024-07-22T08:20:48Z
dc.date.available2024-07-22T08:20:48Z
dc.date.issued2010
dc.description.abstractPropolis is a sticky substance that is collected from plants by honeybees that has anti-mutagenic and anti-carcinogenic properties with biological and therapeutic effects. The target of this study was to investigate the anti-apoptotic effect of propolis extracts (PE) on the caspase pathway in the human breast cell line MCF-7 in culture. Seven different propolis extracts, numbered PE 1-7, produced in their natural ecological environment, were collected from the Hacettepe University Beytepe Campus area in Ankara, Turkey. Individual extracts at 0.5, 0.25, 0.125 and 0.063 mg/ml were incubated with MCF-7 cells during 2 days culture. Cell growth and cytotoxicity were measured colorimetrically by MTT assay. Apoptotic cell death was determined by the TUNEL method (terminal deoxynucleotidyltransferase-biotin nick end-labelling) and caspase activity was investigated by immunocytochemistry using antibodies directed against caspase 6, caspase 8 and caspase 9. The results showed that the PE 5 and 6 extracts at 0.125 mg/ml dilution induced apoptosis in association with increased number of TUNEL positive cells. MTT results showed that cultures exposed to the same extracts and at the same dilution experienced better cell growth compared to those cultures exposed to the other extracts. Immunpositivity for all caspases was detected after treatment with all the extracts and at all dilutions, with stronger immunoreactivity for caspase 6 than caspases 8 and 9. Caspase 6 labelling was especially strong in PE 5 and PE 6. We conclude that propolis may have anti-tumour effects by increasing apoptosis through the caspase pathway. Such propolis extracts may be important economically and allow development of a relatively inexpensive cancer treatment. © 2009 Elsevier GmbH.
dc.identifier.DOI-ID10.1016/j.acthis.2009.06.001
dc.identifier.issn16180372
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18337
dc.language.isoEnglish
dc.subjectAntineoplastic Agents, Phytogenic
dc.subjectApoptosis
dc.subjectBreast Neoplasms
dc.subjectCaspases
dc.subjectCell Proliferation
dc.subjectCell Survival
dc.subjectDose-Response Relationship, Drug
dc.subjectDrug Screening Assays, Antitumor
dc.subjectEnzyme Activation
dc.subjectGas Chromatography-Mass Spectrometry
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectPlant Extracts
dc.subjectPropolis
dc.subjectTumor Cells, Cultured
dc.subjectTurkey
dc.subjectalcohol
dc.subjectcaspase
dc.subjectcaspase 6
dc.subjectcaspase 8
dc.subjectcaspase 9
dc.subjectenzyme antibody
dc.subjectpropolis
dc.subjectantineoplastic activity
dc.subjectapoptosis
dc.subjectarticle
dc.subjectbreast carcinoma
dc.subjectcancer therapy
dc.subjectcell growth
dc.subjectcell strain MCF 7
dc.subjectcolorimetry
dc.subjectconcentration response
dc.subjectcontrolled study
dc.subjectdilution
dc.subjectDNA fragmentation
dc.subjectdrug cytotoxicity
dc.subjectenzyme activation
dc.subjectenzyme activity
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunocytochemistry
dc.subjectimmunoreactivity
dc.subjectincubation time
dc.subjectnick end labeling
dc.subjectsignal transduction
dc.subjectTurkey (republic)
dc.titlePropolis from Turkey induces apoptosis through activating caspases in human breast carcinoma cell lines
dc.typeArticle

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