The paracrine immunomodulatory interactions between the human dental pulp derived mesenchymal stem cells and CD4 T cell subsets

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dc.contributor.authorÖzdemir A.T.
dc.contributor.authorÖzgül Özdemir R.B.
dc.contributor.authorKırmaz C.
dc.contributor.authorSarıboyacı A.E.
dc.contributor.authorÜnal Halbutoğlları Z.S.
dc.contributor.authorÖzel C.
dc.contributor.authorKaraöz E.
dc.date.accessioned2024-07-22T08:11:21Z
dc.date.available2024-07-22T08:11:21Z
dc.date.issued2016
dc.description.abstractMesenchymal stem cells (MSCs) have strong immunomodulatory properties, however these properties may show some differences according to the tissue type of their isolate. In this study we investigated the paracrine interactions between human DP derived MSCs (hDP-MSCs) and the CD4+ T helper cell subsets to establish their immunomodulatory mechanisms. We found that the CD4+-Tbet+ (Th1) and CD4+-Gata3+ (Th2) cells were suppressed by the hDP-MSCs, but the CD4+-Stat3+ (Th17) and CD4+-CD25+-FoxP3+ (Treg) cells were stimulated. The expressions of T cell specific cytokines interferon gamma (IFN-g), interleukin (IL)-4 and IL-17a decreased, but IL-10 and transforming growth factor beta-1 (TGF-b1) increased with the hDP-MSCs. The expressions of indoleamine-pyrrole 2,3-dioxygenase (IDO), prostaglandin E2 (PGE2), soluble human leukocyte antigen G (sHLA-G) derived from hDP-MSCs slightly increased, but hepatocyte growth factor (HGF) significantly increased in the co-culture groups. According to our findings, the hDP-MSCs can suppress the Th1 and Th2 subsets but stimulate the Th17 and Treg subsets. The Stat3 expression of Th17 cells may have been stimulated by the HGF, and thus the pro-inflammatory Th17 cells may have altered into the immunosuppressive regulatory Th17 cells. Further prospective studies are needed to confirm our findings. © 2016 Elsevier Inc.
dc.identifier.DOI-ID10.1016/j.cellimm.2016.08.008
dc.identifier.issn00088749
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15623
dc.language.isoEnglish
dc.publisherAcademic Press Inc.
dc.subjectCell Differentiation
dc.subjectCells, Cultured
dc.subjectDental Pulp
dc.subjectDinoprostone
dc.subjectForkhead Transcription Factors
dc.subjectGATA3 Transcription Factor
dc.subjectHepatocyte Growth Factor
dc.subjectHumans
dc.subjectImmunomodulation
dc.subjectIndoleamine-Pyrrole 2,3,-Dioxygenase
dc.subjectMesenchymal Stromal Cells
dc.subjectParacrine Communication
dc.subjectSTAT3 Transcription Factor
dc.subjectT-Box Domain Proteins
dc.subjectT-Lymphocytes, Regulatory
dc.subjectTh1 Cells
dc.subjectTh17 Cells
dc.subjectTh2 Cells
dc.subjectCD4 antigen
dc.subjectgamma interferon
dc.subjectHLA G antigen
dc.subjectindoleamine 2,3 dioxygenase
dc.subjectinterleukin 10
dc.subjectinterleukin 17
dc.subjectinterleukin 2 receptor alpha
dc.subjectinterleukin 4
dc.subjectprostaglandin E2
dc.subjectscatter factor receptor
dc.subjecttranscription factor FOXP3
dc.subjecttranscription factor GATA 3
dc.subjecttransforming growth factor beta1
dc.subjectforkhead transcription factor
dc.subjectFOXP3 protein, human
dc.subjectGATA3 protein, human
dc.subjectHGF protein, human
dc.subjectindoleamine 2,3 dioxygenase
dc.subjectprostaglandin E2
dc.subjectscatter factor
dc.subjectSTAT3 protein
dc.subjectT box transcription factor
dc.subjectT-box transcription factor TBX21
dc.subjecttranscription factor GATA 3
dc.subjectadaptive immunity
dc.subjectadult
dc.subjectArticle
dc.subjectblood sampling
dc.subjectCD4+ T lymphocyte
dc.subjectcell interaction
dc.subjectcell isolation
dc.subjectcell stimulation
dc.subjectcoculture
dc.subjectcontrolled study
dc.subjecteffector cell
dc.subjectfemale
dc.subjectflow cytometry
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunomodulation
dc.subjectlymphocyte differentiation
dc.subjectlymphocyte proliferation
dc.subjectmesenchymal stem cell
dc.subjectnormal human
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectregulatory T lymphocyte
dc.subjectT lymphocyte subpopulation
dc.subjectTh1 cell
dc.subjectTh17 cell
dc.subjectTh2 cell
dc.subjecttooth pulp
dc.subjectyoung adult
dc.subjectcell culture
dc.subjectcell differentiation
dc.subjectimmunology
dc.subjectimmunomodulation
dc.subjectmesenchymal stroma cell
dc.subjectmetabolism
dc.subjectparacrine signaling
dc.subjectpathology
dc.subjectphysiology
dc.titleThe paracrine immunomodulatory interactions between the human dental pulp derived mesenchymal stem cells and CD4 T cell subsets
dc.typeArticle

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