The glycosylation status of murin postnatal thymus: A study by histochemistry and lectin blotting

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dc.contributor.authorBalcan E.
dc.contributor.authorGümüş A.
dc.contributor.authorŞahin M.
dc.date.accessioned2024-07-22T08:22:20Z
dc.date.available2024-07-22T08:22:20Z
dc.date.issued2008
dc.description.abstractDuring the intrathymic development, the fate of the thymocytes depends largely on variable expression of CD4/CD8 markers and T cell receptor protein expressions. In addition, changes of cell surface glycosylation status also affect the thymocyte maturation. In this study the glycosylation alterations in thymic tissues from 1, 9, 13 and 16 days old mice were evaluated by histochemical and lectin blotting techniques. With alcian blue (AB) at pH 5.7/periodic acid-Schiff (PAS) stainings, it was shown that thymic microenvironments contained carboxlylated and sulfated glycosaminoglycans (GAGs). Strong positivity to AB at pH 2.5, which specific for sialomucins, was seen in some medullary thymocytes. Similarly, it was shown that with Maackia amurensis agglutinin (MAL) medullary thymocytes, but not cortical ones, contained α(2 → 3) linked sialic acid structures. On the other hand, while reaction with peanut agglutinin (PNA), which specific for core disaccharide galactose β(1 → 3) N-acetylgalactosamine, was only seen in cortical thymocytes, reaction with Galanthus nivalis agglutinin (GNA), which specific for terminal mannose residues, was seen in both cortex and medulla. However, Datura stramonium agglutinin (DSA), which recognizes galactose β(1 → 4) N-acetylglucosamine, was not only cell-specific, but it was bound some thymic vessels. With lectin blotting studies, five glycoprotein bands of molecular weights ∼39, ∼54, 100, ∼110 and ∼212 were found which reacted with MAL, PNA and DSA as well as GNA. These results suggest that glycosylation patterns of cell surface glycoconjugates are modified during thymocyte selection processes of postnatal days. © 2008 Springer Science+Business Media B.V.
dc.identifier.DOI-ID10.1007/s10735-008-9180-3
dc.identifier.issn15672379
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19042
dc.language.isoEnglish
dc.subjectAlcian Blue
dc.subjectAnimals
dc.subjectAnimals, Newborn
dc.subjectFemale
dc.subjectGlycosylation
dc.subjectLectins
dc.subjectMale
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectThymus Gland
dc.subjectArachis hypogaea
dc.subjectDatura stramonium
dc.subjectGalanthus nivalis
dc.subjectMaackia amurensis
dc.subjectMus
dc.subjectagglutinin
dc.subjectalcian blue
dc.subjectdisaccharide
dc.subjectdisaccharide galactose beta
dc.subjectglycoprotein
dc.subjectglycosaminoglycan
dc.subjectmannose
dc.subjectn acetylgalactosamine
dc.subjectpeanut agglutinin
dc.subjectsialic acid
dc.subjectsialomucin
dc.subjectlectin
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectblotting
dc.subjectcontrolled study
dc.subjectfemale
dc.subjectglycosylation
dc.subjecthistochemistry
dc.subjectlectin blotting
dc.subjectmale
dc.subjectmouse
dc.subjectnewborn
dc.subjectnonhuman
dc.subjectperinatal period
dc.subjectperiodic acid Schiff stain
dc.subjectpriority journal
dc.subjectthymocyte
dc.subjectthymus
dc.subjectanimal
dc.subjectBagg albino mouse
dc.subjectcytology
dc.subjectglycosylation
dc.subjectmetabolism
dc.titleThe glycosylation status of murin postnatal thymus: A study by histochemistry and lectin blotting
dc.typeArticle

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