Evaluation of xenon, light-emitting diode (LED) and halogen light toxicity on cultured retinal pigment epithelial cells

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dc.contributor.authorSezer T.
dc.contributor.authorAltinisik M.
dc.contributor.authorGuler E.M.
dc.contributor.authorKocyigit A.
dc.contributor.authorOzdemir H.
dc.contributor.authorKoytak A.
dc.date.accessioned2024-07-22T08:08:45Z
dc.date.available2024-07-22T08:08:45Z
dc.date.issued2019
dc.description.abstractObjective: To compare the possible toxic effects of three light sources used in vitreoretinal endoillumination systems; halogen, xenon, and light-emitting diode (LED) on retinal pigment epithelium (RPE) cell cultures, after two different exposure times. Material and methods: ARPE-19 human RPE cell cultures were exposed to halogen, xenon, and LED light sources at a distance of 1.5 cm for 30 and 60 min with equal lumen output levels. Cells in the control group were not exposed. RPE cell cultures were compared in terms of cell viability, DNA damage, apoptosis rate, and IL-1ß, IL-6, and TNF- α levels. Results: The halogen light group showed significantly more DNA damage, higher TNF-α, IL-1β, and IL-6 levels, and lower viable cell count at 30 min compared to the control group. The rates of early and late apoptosis were also significantly higher at 60 min. There were no statistically significant differences in any of the parameters between the xenon and LED light sources and the control group at 30 or 60 min. Conclusion: New generation lights, xenon, and LED, seem to be safe in terms of RPE cells. Halogen light may cause toxic effects on RPE cells when used for a long time with maximal power output. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.
dc.identifier.DOI-ID10.1080/15569527.2018.1539008
dc.identifier.issn15569527
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14521
dc.language.isoEnglish
dc.publisherTaylor and Francis Ltd
dc.subjectApoptosis
dc.subjectCell Line
dc.subjectCytokines
dc.subjectDNA Damage
dc.subjectEpithelial Cells
dc.subjectHalogens
dc.subjectHumans
dc.subjectLight
dc.subjectRetina
dc.subjectRetinal Pigments
dc.subjectXenon
dc.subjecthalogen
dc.subjectinterleukin 1
dc.subjectinterleukin 1beta
dc.subjectinterleukin 6
dc.subjecttumor necrosis factor
dc.subjectxenon
dc.subjectcytokine
dc.subjecthalogen
dc.subjectvisual pigment
dc.subjectxenon
dc.subjectapoptosis
dc.subjectapoptosis rate
dc.subjectARPE-19 cell line
dc.subjectArticle
dc.subjectcell count
dc.subjectcell culture
dc.subjectcell viability
dc.subjectcomparative study
dc.subjectcontrolled study
dc.subjectDNA damage
dc.subjectevaluation study
dc.subjectflow cytometry
dc.subjecthuman
dc.subjecthuman cell
dc.subjectillumination
dc.subjectlight
dc.subjectretinal pigment epithelium
dc.subjecttoxicity
dc.subjectcell line
dc.subjectepithelium cell
dc.subjectlight
dc.subjectmetabolism
dc.subjectradiation response
dc.subjectretina
dc.titleEvaluation of xenon, light-emitting diode (LED) and halogen light toxicity on cultured retinal pigment epithelial cells
dc.typeArticle

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