Multicenter evaluation of crystal violet decolorization assay (CVDA) for rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis

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dc.contributor.authorCoban A.Y.
dc.contributor.authorAkbal A.U.
dc.contributor.authorBicmen C.
dc.contributor.authorAlbay A.
dc.contributor.authorSig A.K.
dc.contributor.authorUzun M.
dc.contributor.authorSelale D.S.
dc.contributor.authorOzkutuk N.
dc.contributor.authorSurucuoglu S.
dc.contributor.authorAlbayrak N.
dc.contributor.authorUcarman N.
dc.contributor.authorOzkutuk A.
dc.contributor.authorEsen N.
dc.contributor.authorCeyhan I.
dc.contributor.authorOzyurt M.
dc.contributor.authorBektore B.
dc.contributor.authorAslan G.
dc.contributor.authorDelialioğlu N.
dc.contributor.authorAlp A.
dc.date.accessioned2024-07-22T08:11:22Z
dc.date.available2024-07-22T08:11:22Z
dc.date.issued2016
dc.description.abstractThe aim of this multicenter study was to evaluate the performance of the crystal violet decolorization assay (CVDA) for detection of multidrug resistant tuberculosis (MDR-TB). This study was performed in 11 centers in two phases. A total of 156 isolates were tested for INH and RIF resistance. In the phase I, 106 clinical isolates were tested in the Center 1–7. In the phase 2, 156 clinical isolates were tested in the center 1–6, center 8–11. Eighty six of 156 tested isolates were the same in phase I. Agreements were 96.2–96.8% for INH and 98.1–98.7% for RIF in the phase I-II, respectively. Mean time to obtain the results in the phase I was 14.3 ± 5.4 days. In the phase II, mean time to obtain the results was 11.6 ± 3.5 days. Test results were obtained within 14days for 62.3% (66/106) of isolates in the phase I and 81.4% (127/156) of isolates in the phase II. In conclusion, CVDA is rapid, reliable, inexpensive, and easy to perform for rapid detection of MDR-TB isolates. In addition, it could be adapted for drug susceptibility testing with all drugs both in developed and developing countries. © 2016, The Author(s).
dc.identifier.DOI-ID10.1038/srep39050
dc.identifier.issn20452322
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15630
dc.language.isoEnglish
dc.publisherNature Publishing Group
dc.rightsAll Open Access; Gold Open Access
dc.subjectCalorimetry
dc.subjectDeveloped Countries
dc.subjectGentian Violet
dc.subjectHumans
dc.subjectIsoniazid
dc.subjectMicrobial Sensitivity Tests
dc.subjectMycobacterium tuberculosis
dc.subjectRifampin
dc.subjectSensitivity and Specificity
dc.subjectTime Factors
dc.subjectTuberculosis, Multidrug-Resistant
dc.subjectcrystal violet
dc.subjectisoniazid
dc.subjectrifampicin
dc.subjectcalorimetry
dc.subjectclinical trial
dc.subjectdeveloped country
dc.subjectdrug effect
dc.subjecthuman
dc.subjectisolation and purification
dc.subjectmicrobial sensitivity test
dc.subjectmulticenter study
dc.subjectmultidrug resistant tuberculosis
dc.subjectMycobacterium tuberculosis
dc.subjectsensitivity and specificity
dc.subjecttime factor
dc.titleMulticenter evaluation of crystal violet decolorization assay (CVDA) for rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis
dc.typeArticle

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