Detection of Leishmania RNA virus 2 in Leishmania species from Turkey

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dc.contributor.authorNalçacı M.
dc.contributor.authorKarakuş M.
dc.contributor.authorYılmaz B.
dc.contributor.authorDemir S.
dc.contributor.authorÖzbilgin A.
dc.contributor.authorÖzbel Y.
dc.contributor.authorTöz S.
dc.date.accessioned2024-07-22T08:08:31Z
dc.date.available2024-07-22T08:08:31Z
dc.date.issued2019
dc.description.abstractBackground: Leishmania RNA virus (LRV) is a double-stranded RNA (dsRNA) virus infecting some Leishmania strains and triggering a destructive hyperinflammatory response in mammalian hosts in the New World. There is limited knowledge of the presence of this virus in Old World Leishmania species and its role in the outcome of the disease. We aimed to investigate the presence of LRV in Leishmania species/strains from Turkey. Methods: Twenty-nine previously identified Leishmania isolates (24 L. tropica, 2 L. infantum, 3 L. major) were examined for LRV positivity using dsRNA visualization in agarose gel after total nucleic acid extraction and RQ-deoxyribonuclease treatment and amplification of a 526 bp fragment of the LRV2-specific RNA-dependent RNA polymerase gene by reverse transcription polymerase chain reaction. Results: Ten (7 L. tropica [24.13%], 3 L. major [10.34%]) of the 29 Leishmania strains gave positive results for LRV. Basic Local Alignment Search Tool analysis showed that all these viruses are LRV2-1. LRV2 was detected for the first time in L. tropica strains in the present study. Conclusions: The clinical manifestation and resistance status of the disease can be different depending on the host and parasite species/strains. The presence of LRV2 may be one of the factors contributing the course of disease. Further studies are needed to elucidate the specific role of LRV2, as it may be a potential target for effective treatment strategies. © The Author(s) 2019. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. All rights reserved.
dc.identifier.DOI-ID10.1093/trstmh/trz023
dc.identifier.issn00359203
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14429
dc.language.isoEnglish
dc.publisherOxford University Press
dc.subjectHumans
dc.subjectLeishmania
dc.subjectLeishmaniasis
dc.subjectLeishmaniavirus
dc.subjectPhylogeny
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectRNA Viruses
dc.subjectSequence Analysis, DNA
dc.subjectTurkey
dc.subjectagarose
dc.subjectdeoxyribonuclease
dc.subjectRNA directed RNA polymerase
dc.subjectArticle
dc.subjectcontrolled study
dc.subjectdouble-stranded RNA virus
dc.subjectgene amplification
dc.subjectgene sequence
dc.subjectLeishmania infantum
dc.subjectLeishmania major
dc.subjectLeishmania RNA virus 2
dc.subjectLeishmania tropica
dc.subjectnonhuman
dc.subjectphylogenetic tree
dc.subjectreverse transcription polymerase chain reaction
dc.subjectTurkey (republic)
dc.subjectvirus detection
dc.subjectDNA sequence
dc.subjectgenetics
dc.subjecthuman
dc.subjectisolation and purification
dc.subjectLeishmania
dc.subjectleishmaniasis
dc.subjectLeishmaniavirus
dc.subjectphylogeny
dc.subjectRNA virus
dc.subjectturkey (bird)
dc.subjectvirology
dc.titleDetection of Leishmania RNA virus 2 in Leishmania species from Turkey
dc.typeArticle

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