Long-term storage of microalgae: determination of optimum cryopreservation conditions

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dc.contributor.authorDeniz I.
dc.contributor.authorDemirel Z.
dc.contributor.authorImamoglu E.
dc.contributor.authorConk-Dalay M.
dc.date.accessioned2024-07-22T08:04:31Z
dc.date.available2024-07-22T08:04:31Z
dc.date.issued2022
dc.description.abstractMaintenance of eukaryotic microalgae strains for the long term is generally carried out using serial subculture techniques which require labour, time and cost. Cryopreservation techniques provide long-term storage of up to years for numerous microorganism strains and cell cultures. Ssu930ijn vbvbhnn8;l,n is related to a successfully designed mass and heat transfer balance throughout the cell. In this study, optimization of the cryopreservation process was carried out for two commercially used microalgal strains. The parameters to be optimized were DMSO percentage (0-25%), incubation time (1-15 min) and cryopreservation term (7-180 days) using a central composite design (CCD). Long-term storage up to 123.17 and 111.44 days corresponding to high cell viabilities was achieved for Chlorella vulgaris and Neochloris texensis, respectively. Generated models were found to be in good agreement with experimental results. The study also revealed holistic results for storage of microalgal strains in a stable state for industrial applications. Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of Marine Biological Association of the United Kingdom.
dc.identifier.DOI-ID10.1017/S0025315422000479
dc.identifier.issn00253154
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12732
dc.language.isoEnglish
dc.publisherCambridge University Press
dc.subjectcell
dc.subjectcryopreservation
dc.subjectdesign
dc.subjecteukaryote
dc.subjectgreen alga
dc.subjectheat transfer
dc.subjectmicroalga
dc.titleLong-term storage of microalgae: determination of optimum cryopreservation conditions
dc.typeArticle

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