The effect of spermatic vessel ligation on testicular nitric oxide levels and germ cell-specific apoptosis in rat testis

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dc.contributor.authorTaneli F.
dc.contributor.authorVatansever S.
dc.contributor.authorUlman C.
dc.contributor.authorYilmaz O.
dc.contributor.authorGiray G.
dc.contributor.authorGenç A.
dc.contributor.authorTaneli C.
dc.date.accessioned2024-07-22T08:24:08Z
dc.date.available2024-07-22T08:24:08Z
dc.date.issued2005
dc.description.abstractManagement of high testis may vary but the most popular method in surgical treatment is the Fowler-Stephens maneuver. The aim of the present study was to investigate the effects of spermatic vessel ligation on testicular nitric oxide (NO) levels, expression of inducible nitric oxide synthase (iNOS), and endothelial nitric oxide synthase (eNOS) and germ cell-specific apoptosis in both ipsilateral and contralateral testes in rats. Twenty-eight animals were randomly allocated into four groups (n=7 each). The spermatic vessels were ligated as a simulation of the Fowler-Stephens maneuver. The groups of animals were sacrificed at 2 h (group 1), 4 h (group 2) and 24 h (group 3) after ligation, respectively. Sham-operated animals served as controls (group 4). Biochemical assessment of testicular NO levels was performed by the Griess method. iNOS and eNOS expression and apoptosis were studied in ipsilateral and contralateral testes. Testicular NO levels at 24 h after the simulated Fowler-Stephens maneuver were found to be significantly increased in both ipsilateral and contralateral testes when compared with the sham-operated group. eNOS expression was clearly increased in ipsilateral testes, whereas moderate expression was detected in the contralateral seminiferous tubules at 24 h after ligation. Mild focal iNOS immunostaining was also observed in seminiferous tubules of the ipsilateral testis at 24 h after the simulated Fowler-Stephens maneuver. Apoptosis was dramatically increased in ipsilateral testes; however, it was only detected in single cells in the contralateral side at 24 h after ligation. In conclusion, the simulated Fowler-Stephens maneuver induces testicular nitric oxide synthesis and germ cell-specific apoptosis in the ipsilateral testis. These results suggest that high levels of NO induce apoptosis and may impair spermatogenesis thus explaining the unsuccessful outcome of the Fowler-Stephens maneuver. © 2004 Elsevier GmbH. All rights reserved.
dc.identifier.DOI-ID10.1016/j.acthis.2004.11.001
dc.identifier.issn00651281
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19851
dc.language.isoEnglish
dc.publisherElsevier GmbH
dc.subjectAnimalia
dc.subjectControl
dc.subjectExperiments
dc.subjectLiving systems studies
dc.subjectNitrogen oxides
dc.subjectSimulation
dc.subjectSurgery
dc.subjectendothelial nitric oxide synthase
dc.subjectnitric oxide
dc.subjectnitric oxide synthase
dc.subjectEndothelial nitric oxide synthase
dc.subjectFowler-Stephens maneuver
dc.subjectGriess method
dc.subjectImmunostaining
dc.subjectInducible nitric oxide synthase
dc.subjectSpermatic vessel ligation
dc.subjectTestes
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectapoptosis
dc.subjectarticle
dc.subjectblood vessel
dc.subjectchemical analysis
dc.subjectcontrolled study
dc.subjectFowler Stephens maneuver
dc.subjectgerm cell
dc.subjectimmunohistochemistry
dc.subjectligation
dc.subjectmale
dc.subjectmale genital system surgery
dc.subjectnonhuman
dc.subjectprotein expression
dc.subjectrandomization
dc.subjectrat
dc.subjectseminiferous tubule
dc.subjectsimulation
dc.subjectstatistical analysis
dc.subjectsurgical technique
dc.subjecttestis
dc.subjectMedicine
dc.titleThe effect of spermatic vessel ligation on testicular nitric oxide levels and germ cell-specific apoptosis in rat testis
dc.typeArticle

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