Potential role of chromatin remodeling factor genes in atrophic gastritis/gastric cancer risk
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Date
2018
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Abstract
Background/Aims: Atrophic gastritis (AG), intestinal metaplasia (IM), and Helicobacter pylori (HP) are the risk factors for the development of gastric cancer (GC). Chromatin remodeling is one of the epigenetic mechanisms involved in the carcinogenesis of GC. The purpose of this study was to investigate the expression profiles of defined chromatin remodeling genes in gastric mucosal samples and their values as gastric carcinogenesis biomarkers. Materials and Methods: In total, 95 patients were included in the study. Patients were divided into 3 groups as: GC group (n=34), AG group (n=36), and control group (n=25). AG group was further divided into subgroups based on the presence of HP and IM in gastric mucosa. Chromatin remodeling gene expressions were analyzed using real-time PCR (RT-PCR) array in all groups. Data were evaluated using the RT-qPCR primer assay data analysis software. Results: EED, CBX3, and MTA1 were more overexpressed, whereas ARID1A, ING5, and CBX7 were more underexpressed in the AG and GC groups compared with the controls. No significant differences were observed between the AG and GC groups concerning the expression of these 6 genes, although the fold change levels of these genes in the GC group were well above than in the AG group. EED, CBX3, and MTA1 were significantly more overexpressed in HP- and IM-positive AG subgroup compared with the HP- or IM-negative AG subgroup. Conclusion: In conclusion, our results provide an evidence of epigenetic alterations in AG. Expressions of EED, CBX3, MTA1, ARID1A, ING5, and CBX7 may be considered as promising markers to be used in GC screening for patients with AG. © Copyright 2018.
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Aged , Aged, 80 and over , Biomarkers, Tumor , Case-Control Studies , Chromatin Assembly and Disassembly , Chromosomal Proteins, Non-Histone , Female , Gastric Mucosa , Gastritis, Atrophic , Helicobacter Infections , Helicobacter pylori , Histone Deacetylases , Humans , Intestines , Male , Metaplasia , Middle Aged , Nuclear Proteins , Polycomb Repressive Complex 1 , Polycomb Repressive Complex 2 , Repressor Proteins , Risk Factors , Stomach Neoplasms , Transcription Factors , Tumor Suppressor Proteins , tumor marker , ARID1A protein, human , BMI1 protein , CBX3 protein, human , CBX7 protein, human , EED protein, human , histone deacetylase , ING5 protein, human , Mta1 protein, human , nonhistone protein , nuclear protein , polycomb repressive complex 2 , repressor protein , transcription factor , tumor marker , tumor suppressor protein , adult , ARID1A gene , Article , atrophic gastritis , cancer screening , CBX3 gene , CBX7 gene , chromatin assembly and disassembly , controlled study , EED gene , epigenetics , female , gene , gene expression , gene overexpression , human , human tissue , ING5 gene , major clinical study , male , MTA1 gene , stomach cancer , stomach carcinogenesis , aged , atrophic gastritis , case control study , chromatin assembly and disassembly , genetics , Helicobacter infection , Helicobacter pylori , intestine , metabolism , metaplasia , middle aged , pathology , risk factor , stomach mucosa , stomach tumor , very elderly