Sodium Alginate Microneedle Arrays Mediate the Transdermal Delivery of Bovine Serum Albumin

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dc.contributor.authorDemir Y.K.
dc.contributor.authorAkan Z.
dc.contributor.authorKerimoglu O.
dc.date.accessioned2024-07-22T08:18:30Z
dc.date.available2024-07-22T08:18:30Z
dc.date.issued2013
dc.description.abstractBackground:The "poke and release" strategy for the delivery of macromolecules using polymeric microneedle (MN) is of great importance because it eliminates microneedle reuse, the risks of biohazardous sharps and cross contamination, and it requires no special disposal mechanism. The main objective of this study was the determination of the stability and delivery of bovine serum albumin (BSA) that was transported across human skin via sodium alginate (SA) microneedle arrays (MNs) and SA needle free patches using two different analytical methods.Methodology and Findings:The capability of two analytical methods, the bicinchoninic acid (BCA) assay and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), to precisely detect and quantify BSA within different types of polymeric MNs was assessed. The ex vivo protein release of BSA across dermatomed human abdominal skin from 10 w/w SA MNs was compared to that from needle-free patches using Franz diffusion cells. The developed applicator was mechanically characterized using a Texture Analyzer. The patch mold and its components were fabricated using a rapid prototyping machine.Conclusions/Significance:The BCA method was able to precisely detect BSA that had been loaded into SA MNs. However, the use of SDS-PAGE as the analytical method resulted in significantly different amounts of BSA recovered from differently conditioned polymeric MNs. The permeation of BSA across dermatomed human abdominal skin by SA MNs, which were composed of 100 pyramidal needles, increased by approximately 15.4 fold compared to the permeation obtained with SA needle-free patches. The ease of use of the applicator during the release studies was also demonstrated, as was its mechanical characterization. © 2013 Demir et al.
dc.identifier.DOI-ID10.1371/journal.pone.0063819
dc.identifier.issn19326203
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17326
dc.language.isoEnglish
dc.rightsAll Open Access; Gold Open Access
dc.subjectAdministration, Cutaneous
dc.subjectAlginates
dc.subjectDrug Delivery Systems
dc.subjectDrug Stability
dc.subjectGlucuronic Acid
dc.subjectHexuronic Acids
dc.subjectHumans
dc.subjectMicroinjections
dc.subjectNeedles
dc.subjectPermeability
dc.subjectReproducibility of Results
dc.subjectSerum Albumin, Bovine
dc.subjectTransdermal Patch
dc.subjectalginic acid
dc.subjectbovine serum albumin
dc.subjectaccuracy
dc.subjectanalytic method
dc.subjectarticle
dc.subjectbicinchoninic acid assay
dc.subjectcontrolled study
dc.subjectdissolution
dc.subjectdrug delivery device
dc.subjectex vivo study
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectintermethod comparison
dc.subjectlimit of detection
dc.subjectlimit of quantitation
dc.subjectmicroneedle
dc.subjectmicrotechnology
dc.subjectpolyacrylamide gel electrophoresis
dc.subjectprediction
dc.subjectprotein secretion
dc.subjectprotein transport
dc.subjecttransdermal patch
dc.titleSodium Alginate Microneedle Arrays Mediate the Transdermal Delivery of Bovine Serum Albumin
dc.typeArticle

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